食品科学 ›› 2021, Vol. 42 ›› Issue (5): 39-45.doi: 10.7506/spkx1002-6630-20200303-042

• 基础研究 • 上一篇    下一篇

玉米肽的纳滤脱盐工艺及脱盐产物抗氧化活性

王晓杰,曲悦,刘晓兰,马瑞   

  1. (齐齐哈尔大学食品与生物工程学院,黑龙江省玉米深加工理论与技术重点实验室,黑龙江 齐齐哈尔 161006)
  • 出版日期:2021-03-15 发布日期:2021-03-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400200);黑龙江省自然科学基金联合引导项目(LH2020C111);

Corn Peptide: Desalting by Nanofiltration and Antioxidant Activity

WANG Xiaojie, QU Yue, LIU Xiaolan, MA Rui   

  1. (Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology, College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China)
  • Online:2021-03-15 Published:2021-03-29

摘要: 以具有抗氧化活性的玉米肽为原料,首先采用150 Da纳滤膜对玉米肽进行脱盐处理,通过对膜通量、电导率、Na+质量分数、脱盐率和纳滤后产物抗氧化活性等参数的分析,确定玉米肽的纳滤脱盐工艺,然后利用H2O2诱导的Caco-2细胞氧化损伤模型对脱盐玉米肽的抗氧化活性进行研究。结果表明:利用150 Da纳滤膜对玉米肽脱盐的工艺条件为压力20 bar、频率50 Hz、温度20 ℃、纳滤5 次,每次体积浓缩倍数为2。在此条件下,玉米肽的脱盐率为70.73%,短肽回收率达96.73%,与纳滤前相比,电导率降低了33.86%,玉米肽的1,1-二苯基-2-三硝基苯肼自由基和羟自由基清除率分别提高21.55%和35.93%,而亚铁离子螯合率降低7.38%。质量浓度为75 μg/mL的脱盐玉米肽通过显著提高细胞内的抗氧化酶(超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶和γ-谷氨酰半胱氨酸合成酶)活力而提高由H2O2诱导的氧化性损伤Caco-2细胞的存活率。

关键词: 玉米肽;纳滤;电导率;脱盐率;氧化性损伤

Abstract: In the present study, corn antioxidant peptide was desalted using a nanofiltration membrane with molecular mass cut-off of 150 Da. The desalting process was evaluated with respect to membrane flux, conductivity, Na+ concentration, desalting efficiency and antioxidant of nanofiltration product, and the protective effect of the desalted peptide on H2O2-induced oxidative damage in Caco-2 cell model was determined. The results showed that the desalination conditions were as follows: pressure 20 bar, frequency 50 Hz, temperature 20 ℃, number of nanofiltration cycles 5, and volumetric concentration factor in each nanofiltration 2. Under these conditions, the desalting efficiency was 70.73%, and the recovery of short peptide was 96.73%. Compared to that before nanofiltration, the conductivity was decreased by 33.86%, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical scavenging capacities were increased by 21.55% and 35.93%, respectively, while the ferrous ion chelating capacity was reduced by 7.38%. The desalted corn peptides at 75 μg/mL remarkably increased the viability of H2O2-induced Caco-2 cells by increasing the activity of cellular antioxidant enzymes (superoxide dismutase, glutathione peroxidase, catalase, and γ-glutamylcysteine synthetase).

Key words: corn peptide; nanofiltration; conductivity; desalting efficiency; oxidative injury

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