花椒精油对链脲佐菌素诱导的糖尿病小鼠糖代谢的影响

doi:10.7506/spkx1002-6630-20200424-321

食品科学 ›› 2021, Vol. 42 ›› Issue (9): 115-122.doi: 10.7506/spkx1002-6630-20200424-321

花椒精油对链脲佐菌素诱导的糖尿病小鼠糖代谢的影响

陆敏涛，任廷远，杨建，陆龙发，秦礼康

（1.贵州大学酿酒与食品工程学院，贵州贵阳 550025；2.贵州省农业生物工程重点实验室，贵州贵阳 550025；3.贵州省农业科学院，农业农村部植物新品种测试贵阳分中心，贵州贵阳 550006；4.贵州玄德生物科技股份有限公司，贵州贵阳 550016）

出版日期:2021-05-15 发布日期:2021-06-02
基金资助:
贵州省科技项目（黔科合基础[2020]1Y431；黔科合平台人才[2020]2105；黔科合平台人才[2018]5781-50）；贵州省林业厅科技项目（特林研2020-010）；贵州大学引进人才科研项目（贵大人基合字201842号）

Effect of the Essential Oil from the Ripe Fruit Pericarp of Zanthoxylum bungeanum Maxim. on Glucose Metabolism in Diabetic Mice Induced by Streptozotocin

LU Mintao, REN Tingyuan, YANG Jian, LU Longfa, QIN Likang

(1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China; 2. Guizhou Provincial Key Laboratory of Agricultural Bioengineering, Guiyang 550025, China; 3. Guiyang Station for Testing Center of New Plant Varieties of the Ministry of Agriculture and Rural Affairs, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China; 4. Guizhou Xuande Biotechnology Co. Ltd., Guiyang 550016, China)

Online:2021-05-15 Published:2021-06-02

摘要/Abstract

摘要： 目的：研究花椒精油对1型糖尿病机体糖代谢的影响及可能机制。方法：将50 只健康雄性昆明小鼠（KM小鼠）分为空白对照，糖尿病模型组，花椒精油高（15 mg/（kg mb·d））、中（9 mg/（kg mb·d））、低（3 mg/（kg mb·d））剂量组，每组10 只。采用链脲佐菌素诱导建立1型糖尿病模型。空白组和糖尿病模型组灌胃等剂量的大豆油，给药28 d后，检测血清胰岛素、糖化血清蛋白（glycosylated serum protein，GSP）及糖化血红蛋白（glycosylated hemoglobin，GHb）、肌糖原和肝糖原水平；应用实时荧光定量聚合酶链式反应测定肝脏磷酸肌醇3激酶（phosphoinositide 3-kinase，PI3K）、蛋白激酶B（protein kinase B，PKB）、葡萄糖转运子2（glucose transporter 2，GLUT2）和骨骼肌葡萄糖转运子4（glucose transporter 4，GLUT4）mRNA相对表达量；Western blot检测肝脏PI3K（p110）、PKB和p-PKB（Ser473）的蛋白表达量。实验期间每周测定其空腹血糖浓度及体质量，给药28 d后进行葡萄糖耐量测定。结果：与模型组相比，花椒精油剂量组的摄食量、饮水量、空腹血糖浓度、GHb质量浓度、GSP质量浓度最高分别下降38.91%、35.93%、28.60%、30.76%、37.29%，胰岛素、肝糖原与肌糖原水平最高分别提高22.56%、23.61%、149.75%；PI3K、PKB、GLUT2、GLUT4 mRNA相对表达量最高分别上调87.5%、53.4%、77.05%、133.3%；花椒精油高、中、低剂量组PI3K（p110）蛋白表达量分别上调了3.20、2.51 倍和1.67 倍，p-PKB（Ser473）蛋白表达量分别上调了7.64、6.12 倍和2.73 倍。结论：花椒精油对1型糖尿病小鼠具有降糖效果，其可能机制是激活PI3K/PKB途径，使GLUT2/4移位，促进细胞对葡萄糖的吸收和利用。

关键词: 花椒精油；1型糖尿病；糖代谢；机制

Abstract: Objective: To explore the effect of the essential oil from the ripe fruit percicarp of Zanthoxylum bungeanum Maxim. on glucose metabolism in type I diabetic mice and its possible mechanism. Methods: Totally 50 healthy male Kunming mice were randomly divided into five groups of 10 animals each, including blank control, diabetic model, high-dose (15 mg/(kg mb·d)), middle-dose (9 mg/(kg mb·d)) and low-dose (3 mg/(kg mb·d)) essential oil. Type I diabetes was induced by streptozotocin (STZ). The animals in the blank control and diabetic model groups were orallytreated with the same dose of soybean oil. After 28 days of administration, the levels of insulin, glycosylated serum protein (GSP), glycosylated hemoglobin (GHb), muscle glycogen and liver glycogen were determined. The relative mRNA expression of the phosphoinositide 3-kinase (PI3K), protein kinase B (PKB) and glucose transporter 2 (GLUT2) genes in liver and of the GLUT4 gene in skeletal muscle were determined by quantitative real-time polymerase chain reaction (qRT-PCR ). The protein expression of PI3K (p110), PKB and p-PKB (Ser473) in liver was evaluated by Western blot. Fasting blood glucose and body mass were measured weekly during the experiment, and glucose tolerance was measured on the 28th day of administration. Results: Compared with the model group, food intake, water intake, fasting blood glucose, GHb concentration and GSP concentration in the essential oil treatment groupswere decreased at most by 38.91%, 35.93%, 28.60%, 30.76% and 37.29%, respectively. The levels of insulin, liver glycogen and muscle glycogen were increased at most by 22.56%, 23.61% and 149.75%, respectively. The relative mRNA expression of PI3K, PKB, GLUT2 and GLUT4 was up-regulated (P < 0.05) at most by 87.5%, 53.4%, 77.05% and 133.3%, respectively. Furthermore, the protein expression of PI3K (p110) was up-regulated by 3.20, 2.51 and 1.67 times, and the protein expression of p-PKB (Ser473) was up-regulated by 7.64, 6.12 and 2.73 times in the high-, medium- and low-dose groups, respectively. Conclusion: The essential oil has a hypoglycemic effect in type 1 diabetic mice possibly by activating the PI3K/PKB pathway to translocate GLUT2/4, and promote glucose absorption and utilization by cells.

Key words: essential oil from the ripe fruit percicarp of Zanthoxylum bungeanum Maxim.; type 1 diabetes mellitus; glucose metabolism; mechanism

中图分类号:

R285.5

陆敏涛，任廷远，杨建，陆龙发，秦礼康. 花椒精油对链脲佐菌素诱导的糖尿病小鼠糖代谢的影响[J]. 食品科学, 2021, 42(9): 115-122.

LU Mintao, REN Tingyuan, YANG Jian, LU Longfa, QIN Likang. Effect of the Essential Oil from the Ripe Fruit Pericarp of Zanthoxylum bungeanum Maxim. on Glucose Metabolism in Diabetic Mice Induced by Streptozotocin[J]. FOOD SCIENCE, 2021, 42(9): 115-122.

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花椒精油对链脲佐菌素诱导的糖尿病小鼠糖代谢的影响

Effect of the Essential Oil from the Ripe Fruit Pericarp of Zanthoxylum bungeanum Maxim. on Glucose Metabolism in Diabetic Mice Induced by Streptozotocin

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