壳聚糖处理对西番莲果实感病指数、抗病相关酶活性和抗病物质含量的影响

doi:10.7506/spkx1002-6630-20200723-315

食品科学 ›› 2021, Vol. 42 ›› Issue (15): 206-212.doi: 10.7506/spkx1002-6630-20200723-315

壳聚糖处理对西番莲果实感病指数、抗病相关酶活性和抗病物质含量的影响

郭欣，林育钊，林河通，李倩，段睿琦，朱文婧

（1.漳州职业技术学院食品工程学院，福建漳州 363000；2.福建农林大学食品科学学院，福建福州 350002；3.亚热带特色农产品采后生物学福建省高校重点实验室，福建福州 350002）

出版日期:2021-08-15 发布日期:2021-08-27
基金资助:
福建省财政厅科技项目（KLe16H01A；KLe16002A）；福建省科技计划项目（2018N2002）；福建农林大学科技创新专项基金项目（KF2015051；CXZX2016086）

Effect of Chitosan Treatment on Disease Index, Disease Resistant-Related Enzyme Activities and Disease Resistance-Related Substance Contents in Passiflora caerulea L. Fruit during Storage

GUO Xin, LIN Yuzhao, LIN Hetong, LI Qian, DUAN Ruiqi, ZHU Wenjing

(1. College of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, China;2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou 350002, China)

Online:2021-08-15 Published:2021-08-27

摘要/Abstract

摘要： 为了解壳聚糖处理对西番莲果实采后病害发生的影响效果及其作用机理，采后‘福建百香果1号’西番莲果实经蒸馏水（对照）和稀释200 倍的卡多赞（一种新型壳聚糖）溶液分别浸泡5 min，在（28±1）℃条件下贮藏，每隔3 d测定西番莲果实感病指数、抗病相关酶活力和抗病物质含量的变化。结果发现：与对照组相比，壳聚糖处理能显著降低西番莲果实采后贮藏期间的感病指数（P＜0.05、P＜0.01），贮藏至第15天时，壳聚糖处理的果实感病指数为2.21，仅为对照组果实感病指数（3.99）的55.39%。此外，与对照组比较，壳聚糖处理可保持较高的西番莲果皮总酚和木质素等抗病物质含量，及较高的果皮苯丙氨酸解氨酶、肉桂酸-4-羟化酶、4-香豆酰辅酶A连接酶、肉桂醇脱氢酶、多酚氧化酶、过氧化物酶、几丁质酶和β-1,3葡聚糖酶等抗病相关酶活性。因此认为，壳聚糖处理通过促进西番莲果实抗病物质的生成和积累及提高抗病相关酶活性，从而增强采后西番莲果实的抗病性、抑制果实病害发生，延长果实保鲜期。

关键词: 西番莲果实；采后病害；抗病物质；抗病相关酶；壳聚糖

Abstract: In order to understand the e?ect of chitosan treatment on the postharvest disease development of Passiflora caerulea L. and the underlying mechanisms, freshly harvested fruit (cv. Fujian Baixiangguo 1) were soaked in distilled water (control) and a 1:200 dilution of Kadozan (a novel chitosan formulation) for 5 min, separately, and then stored at (28 ± 1) ℃. Disease index, disease resistance-related enzyme activities and disease resistance-related substance contents were measured every three days. The results indicated that chitosan treatment could significantly reduce disease index of Passiflora caerulea L. fruit during postharvest storage (P < 0.05, P < 0.01); on day 15, the disease index of the treated fruit was 2.21, which was only 55.39% of that (3.99) of the control fruit. In addition, compared with the control fruit, chitosan treated-fruit maintained higher contents of total phenols and lignin, and higher activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), polyphenol oxidase (PPO), peroxidase (POD), chitinase (CHI), and β-1,3-glucanase (GLU) in the pericarp. These findings suggested that chitosan treatment could enhance disease resistance of harvested Passiflora caerulea L. fruit, consequently suppress the development of postharvest diseases and prolong the storage life by promoting the generation and accumulation of disease resistance-related substances and increase the activities of disease defense-related enzymes in the pericarp.

Key words: Passiflora caerulea L. fruit; postharvest disease; disease resistance-related substances; disease resistance-related enzymes; chitosan

中图分类号:

TS255.36

郭欣，林育钊，林河通，李倩，段睿琦，朱文婧. 壳聚糖处理对西番莲果实感病指数、抗病相关酶活性和抗病物质含量的影响[J]. 食品科学, 2021, 42(15): 206-212.

GUO Xin, LIN Yuzhao, LIN Hetong, LI Qian, DUAN Ruiqi, ZHU Wenjing. Effect of Chitosan Treatment on Disease Index, Disease Resistant-Related Enzyme Activities and Disease Resistance-Related Substance Contents in Passiflora caerulea L. Fruit during Storage[J]. FOOD SCIENCE, 2021, 42(15): 206-212.

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壳聚糖处理对西番莲果实感病指数、抗病相关酶活性和抗病物质含量的影响

Effect of Chitosan Treatment on Disease Index, Disease Resistant-Related Enzyme Activities and Disease Resistance-Related Substance Contents in Passiflora caerulea L. Fruit during Storage

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