婴儿肠道源格氏乳杆菌的安全性评价及益生特性

doi:10.7506/spkx1002-6630-20200706-080

食品科学 ›› 2021, Vol. 42 ›› Issue (16): 61-68.doi: 10.7506/spkx1002-6630-20200706-080

婴儿肠道源格氏乳杆菌的安全性评价及益生特性

周钦育，许喜林，赵珊，黄燕燕，邝嘉华，胡金双，刘冬梅

（华南理工大学食品科学与工程学院，广东广州 510641）

发布日期:2021-08-27
基金资助:
国家自然科学基金面上项目（31771908）；广州市科技计划项目（201903010015）

Safety Evaluation and Probiotic Properties of Lactobacillus gasseri Isolated from Intestinal Tract of Infants

ZHOU Qinyu, XU Xilin, ZHAO Shan, HUANG Yanyan, KUANG Jiahua, HU Jinshuang, LIU Dongmei

(School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)

Published:2021-08-27

摘要/Abstract

摘要： 以广州1?月龄婴儿肠道中分离出的乳酸杆菌为实验菌株，经分子生物学鉴定，确认为格氏乳杆菌（Lactobacillus gasseri）LGZ 1029。对格氏乳杆菌LGZ 1029进行体内外安全性评价和益生特性研究，结果表明：格氏乳杆菌LGZ?1029不产溶血毒素，对多类抗生素表现出敏感性，小鼠经口急性无毒；该菌具有较好的胃肠液耐受力及自凝聚能力，疏水性（79.75%）强于商业标准菌株鼠李糖乳杆菌ATCC?7469（43.01%）；菌株对大肠杆菌ATCC?25922抑菌圈直径为17.31～20.70?mm，对金黄色葡萄球菌ATCC?12598的抑菌圈直径为21.71～31.08?mm，抑菌效果强于鼠李糖乳杆菌ATCC?7469；活菌数为1×107?CFU/mL发酵液的1,1-苯基-2-三硝基苯肼自由基清除率可达87.07%。本实验为格氏乳杆菌LGZ?1029在膳食补充剂、乳品制造和防腐剂替代品领域的应用提供理论依据。

关键词: 格氏乳杆菌；婴儿肠道；安全性；益生特性

Abstract: The in vivo and in vitro safety and probiotic properties of Lactobacillus gasseri LGZ 1029, isolated from the intestinal tract of a 1-month-old baby in Guangzhou and identified by molecular biology methods were evaluated. The results showed that L. gasseri LGZ 1029 did not produce hemolytic toxin, and was sensitive to many kinds of antibiotics. It had no acute oral toxicity to mice and had good gastrointestinal tolerance and self-aggregation capacity. Its hydrophobicity (79.75%) was stronger than that (43.01%) of the commercial standard strain L. rhamnosus ATCC 7469. The diameter of inhibition zone of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 12598 when exposed to L. gasseri LGZ1029 was 17.31–20.70 and 21.71–31.08 mm, respectively, and the inhibitory effect was stronger than L. rhamnosus ATCC 7469. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of 1 × 107 CFU/mL L. gasseri LGZ 1029 was determined as 87.07%. These results may provide a theoretical basis for the application of L. gasseri LGZ 1029 in dietary supplements and dairy products manufacturing and its application as a bioprotective agent.

Key words: Lactobacillus gasseri; intestinal tract of infants; safety; probiotic properties

中图分类号:

周钦育，许喜林，赵珊，黄燕燕，邝嘉华，胡金双，刘冬梅. 婴儿肠道源格氏乳杆菌的安全性评价及益生特性[J]. 食品科学, 2021, 42(16): 61-68.

ZHOU Qinyu, XU Xilin, ZHAO Shan, HUANG Yanyan, KUANG Jiahua, HU Jinshuang, LIU Dongmei. Safety Evaluation and Probiotic Properties of Lactobacillus gasseri Isolated from Intestinal Tract of Infants[J]. FOOD SCIENCE, 2021, 42(16): 61-68.

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婴儿肠道源格氏乳杆菌的安全性评价及益生特性

Safety Evaluation and Probiotic Properties of Lactobacillus gasseri Isolated from Intestinal Tract of Infants

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