基于氮阻遏效应分析汉逊德巴利酵母组胺调控

doi:10.7506/spkx1002-6630-20201026-255

食品科学 ›› 2022, Vol. 43 ›› Issue (2): 93-99.doi: 10.7506/spkx1002-6630-20201026-255

基于氮阻遏效应分析汉逊德巴利酵母组胺调控

王新惠，潘攀，孙劲松，丁悦，刘力嘉，张雅琳，肖龙泉，田甜，刘洋

（1.成都大学食品与生物工程学院，肉类加工四川省重点实验室，四川成都 610106；2.宜宾学院固态发酵资源利用四川省重点实验室，四川宜宾 644000；3.成都农业科技职业学院，四川成都 611130）

出版日期:2022-01-25 发布日期:2022-01-29
基金资助:
国家自然科学基金面上项目（31772093）；四川省科技厅应用基础项目（2018JY0365）；四川省科技创新苗子工程项目（2020058）

Analysis of Histamine Regulation in Debaryomyces hansenii Based on Nitrogen Catabolite Repression

WANG Xinhui, PAN Pan, SUN Jinsong, DING Yue, LIU Lijia, ZHANG Yalin, XIAO Longquan, TIAN Tian, LIU Yang

(1. Key Laboratory of Meat Processing of Sichuan, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China; 2. Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin 644000, China; 3. Chengdu Agricultural College, Chengdu 611130, China)

Online:2022-01-25 Published:2022-01-29

摘要/Abstract

摘要： 研究添加L-组氨酸和不添加L-组氨酸两种不同氮源水平情况下，汉逊德巴利酵母（Debaryomyces hansenii）氮代谢阻遏效应调控机制。以添加L-组氨酸为实验组，不添加组为对照，利用Illumina高通量测序平台对两组进行转录组测序，通过多种生物信息学方法对数据进行分析处理。对照组测序结果共得到42.921 6 M clean reads；实验组测序结果共得到41.415 4 M clean reads。两组样品分析显示共有133 个显著差异表达基因，78 个基因表达上调，50 个基因表达下调，基因本体论（Gene Ontology，GO）和京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析表明，差异表达基因参与了组胺代谢相关酶系代谢。本研究为汉逊德巴利酵母组胺代谢进一步研究提供了参考。

关键词: 汉逊德巴利酵母；转录组；氮代谢阻遏效应；组胺

Abstract: This study was performed in order to explore the regulatory mechanism of nitrogen catabolite repression (NCR) in Debaryomyces hansenii cultured under two different nitrogen levels: with (experimental group) and without (control group) L-histidine addition. The Illumina high-throughput sequencing platform was used for transcriptome sequencing of both groups, and various bioinformatics methods were used for data analysis and processing. A total of 42.921 6 and 41.415 4 M clean reads were obtained for the control and experimental groups. Furthermore, a total of 133 significantly differentially expressed genes were identified between the two groups, 78 of which were up-regulated, while 50 were down-regulated. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that most of the differentially expressed genes were involved in histamine metabolism-related coenzymes. This study provides a reference for further studies on histamine metabolism in D. hansenii.

Key words: Debaryomyces hansenii; transcriptome; nitrogen catabolite repression; histamine

中图分类号:

TS251.65

王新惠，潘攀，孙劲松，丁悦，刘力嘉，张雅琳，肖龙泉，田甜，刘洋. 基于氮阻遏效应分析汉逊德巴利酵母组胺调控[J]. 食品科学, 2022, 43(2): 93-99.

WANG Xinhui, PAN Pan, SUN Jinsong, DING Yue, LIU Lijia, ZHANG Yalin, XIAO Longquan, TIAN Tian, LIU Yang. Analysis of Histamine Regulation in Debaryomyces hansenii Based on Nitrogen Catabolite Repression[J]. FOOD SCIENCE, 2022, 43(2): 93-99.

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基于氮阻遏效应分析汉逊德巴利酵母组胺调控

Analysis of Histamine Regulation in Debaryomyces hansenii Based on Nitrogen Catabolite Repression

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