春夏季茶树儿茶素生物合成基因表达差异

doi:10.7506/spkx1002-6630-20210113-150

食品科学 ›› 2022, Vol. 43 ›› Issue (4): 80-87.doi: 10.7506/spkx1002-6630-20210113-150

春夏季茶树儿茶素生物合成基因表达差异

黄芳芳，熊立瑰，李莹，刘仲华，黄建安，李娟

（1.湖南农业大学茶学教育部重点实验室，湖南长沙 410128；2.国家植物功能成分利用工程技术研究中心，湖南长沙 410128；3.湖南省植物功能成分利用省部共建协同创新中心，湖南长沙 410128）

出版日期:2022-02-25 发布日期:2022-03-08
基金资助:
国家自然科学基金联合基金项目（U19A2030）；国家自然科学基金面上项目（32072629）；国家自然科学基金青年科学基金项目（31500567）

Differential Gene Expression Involved in Catechin Synthesis of Tea Leaves (Camellia sinensis) during Spring and Summer

HUANG Fangfang, XIONG Ligui, LI Ying, LIU Zhonghua, HUANG Jian’an, LI Juan

(1. Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China; 2. National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China; 3. Hunan Co-innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Changsha 410128, China)

Online:2022-02-25 Published:2022-03-08

摘要/Abstract

摘要： 以福鼎大白茶、白毫早、碧香早、槠叶齐一芽二叶为材料，采用GB/T 8313—2018《茶叶中茶多酚和儿茶素类含量的检测方法》测定茶多酚含量，高效液相色谱测定儿茶素组分含量，采用实时聚合酶链式反应测定儿茶素生物合成基因相对表达量。结果表明：不同茶树品种表没食子儿茶素、表儿茶素没食子酸酯、表儿茶素的含量及茶多酚总量夏季均极显著高于春季（P＜0.01）。黄烷酮3-羟化酶基因、二氢黄酮醇4-还原酶基因的表达量有春季高于夏季的趋势；春季采摘的白毫早样品花青素合成酶基因、肉桂酸羟化酶基因、查耳酮合成酶基因、UDP-糖基转移酶基因、类黄酮3’,5’-羟化酶（flavonoid 3’,5’-hydroxylase，F3’5’H）基因相对表达量与其他样品均存在差异（|log2差异倍数|≥1），由此可知基因的表达量不仅受季节调控，还受到品种的影响。F3’5’H基因相对表达量与表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）含量存在正相关，EGCG是黄烷酮B环3’,4’,5’位置羟基化物质，催化其羟基化的酶为类黄酮3’-羟化酶和F3’5’H，因此F3’5’H可能是EGCG合成的关键酶。

关键词: 春夏季；茶树；儿茶素含量；基因相对表达量

Abstract: In this study, the contents of polyphenols and catechins in the spring and summer shoots with one bud and two leaves of the tea cultivars Fuding Dabaicha, Baihaozao, Bixiangzao and Zhuyeqi were determined by the method specified in the national standard GB/T 8313-2018 and high-performance liquid chromatography (HPLC). The relative expression levels of catechin biosynthesis-related genes were determined by real-time polymerase chain reaction (real-time PCR). The results showed that for the four cultivars, the contents of EGC, ECG and EC in summer tea shoots were significantly higher than those in spring tea shoots (P < 0.01). The expression levels of the F3H and DFR genes tended to be higher in spring than in summer. Differential expression levels (|log2 fold change|≥1) of the ANS, C4H, CHS, ANR, UGT84A and flavonoid 3’,5’-hydroxylase (F3’5’H) genes were found between spring tea shoots from Baihaozao and other samples, implying that the gene expression levels could be not only regulated by season, but also affected by cultivar. The relative expression level of F3’5’H was positively correlated with the content of EGCG, which is derived from the hydroxylation of flavanone at the 3’,4’, and 5’ positions of the B ring catalyzed by flavonoid 3’-hydroxylase (F3’H) and F3′5′H. Therefore, F3’5’H may be the key enzyme for EGCG synthesis.

Key words: spring and summer; tea; contents of catechin; relative gene expression

中图分类号:

S571.1

黄芳芳，熊立瑰，李莹，刘仲华，黄建安，李娟. 春夏季茶树儿茶素生物合成基因表达差异[J]. 食品科学, 2022, 43(4): 80-87.

HUANG Fangfang, XIONG Ligui, LI Ying, LIU Zhonghua, HUANG Jian’an, LI Juan. Differential Gene Expression Involved in Catechin Synthesis of Tea Leaves (Camellia sinensis) during Spring and Summer[J]. FOOD SCIENCE, 2022, 43(4): 80-87.

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春夏季茶树儿茶素生物合成基因表达差异

Differential Gene Expression Involved in Catechin Synthesis of Tea Leaves (Camellia sinensis) during Spring and Summer

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