黑色素-纳米硒的制备及其性质

摘要/Abstract

摘要： 本论文以黑芝麻黑色素为模板剂和还原剂制备黑色素-纳米硒，对黑色素-纳米硒的制备工艺进行优化，并表征其结构和形貌，之后进一步探讨其缓释特性、体外抗氧化能力，评价其对红细胞和内皮细胞EAhy926氧化损伤的保护作用，通过肺癌细胞A549模型和海虾致死实验探讨其抑癌活性和生物相容性。结果表明黑色素-纳米硒合成的最适条件为：黑色素溶液（0.3 mg/mL）与二氧化硒溶液（0.555 mg/mL）体积比为9:5，反应pH为7，40 ℃避光磁力搅拌（400 r/min）反应5 h。X 射线光电子能谱结果表明Se4+可被全部还原。红外结果表明黑色素结构中羧基及共轭结构可能参与了反应。同步光散射仪表征黑色素-纳米硒粒径约为200 nm，性质稳定，能较好的分布于水分散体系中。黑色素-纳米硒在人工模拟胃液环境中可稳定存在2 h，具有一定的pH稳定性，在人工模拟肠液环境中可缓慢释放，能实现在肠液中的长效缓释。体外抗氧化结果表明黑色素-纳米硒对ABTS+自由基的清除率和总还原力显著优于二氧化硒。黑色素-纳米硒能显著抑制过氧化氢引发的红细胞溶血和内皮细胞EAhy926的氧化损伤。黑色素-纳米硒对肺癌细胞具有一定的抑制活性。海虾致死实验结果表明黑色素-纳米硒（半致死浓度为347.393 μg/mL）具有较二氧化硒（半致死浓度为19.781 μg/mL）较弱毒性。本研究为黑色素资源的深入研究和黑色素-纳米硒的制备提供了一定的指导作用，也为黑色素-纳米硒用作膳食补硒剂提供了一定的理论基础。

关键词: 黑色素-纳米硒, 缓释性能, 抗氧化, 抑癌活性

Abstract: In this paper, black sesame melanin was used as template and reducing agent to prepare melanin-nano-selenium. The preparation process of melanin-nano-selenium was optimized, and its structure and morphology were characterized. After that, its antioxidant capacity in vitro was further discussed, and its protective effect on oxidative damage of red blood cell and endothelial cell EAhy926 was evaluated. The anticancer activity and biocompatibility of melanin-nano-selenium were investigated by lung cancer cell A549 model and marine shrimp lethal experiment. The results showed that the optimum conditions for the synthesis of melanin-nano-selenium were as follows: the volume ratio of melanin solution (0.3 mg/mL) to selenium dioxide solution (0.555 mg/mL) was 9:5, the pH was 7, and the reaction time was 5 h by magnetic stirring (400 r/min). The results of X-ray photoelectron spectroscopy showed that Se4+ could be completely reduced. The IR results indicated that the carboxyl and conjugate structures of melanin might be involved in the reaction. The particle size of melanin-nano-selenium is about 200 nm, which is stable and can be well distributed in water dispersion system. melanin-nano-selenium can stably exist for 2 h in artificial simulated gastric juice environment, and has a certain pH stability. It can be released slowly in artificial simulated intestinal juice environment, and can achieve long-term sustained release in intestinal juice. In vitro antioxidant results showed that the scavenging rate and total reducing power of melanin-nano-selenium on ABTS+ radical were significantly better than that of selenium dioxide. melanin-nano-selenium significantly inhibited hemolysis of red blood cells and oxidative damage of endothelial cells EAhy926 induced by hydrogen peroxide. melanin-nano-selenium has certain inhibitory activity on lung cancer cells. The toxicity of melanin-nano-selenium (lethal concentration 347.393 μg/mL) was weaker than that of selenium dioxide (lethal concentration 19.781 μg/mL). This study provides some guidance for the further study of melanin resources and the preparation of melanin-nano-selenium, and also provides some theoretical basis for the use of melanin-nano-selenium as dietary selenium supplement.

Key words: melanin-nano-selenium, sustained release performance, antioxidant, anticancer activity

中图分类号:

高莉任燕玲刘琳琳徐宏宇郝瑞赵英虎. 黑色素-纳米硒的制备及其性质[J]. 食品科学.

Liu 琳琳刘 Linlin Liu. Preparation and properties of melanin-nano-selenium[J]. FOOD SCIENCE.

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