微量元素硼对大鼠回肠显微结构、消化酶活性、屏障功能及抗氧化功能的影响

doi:10.7506/spkx1002-6630-20210517-191

食品科学 ›› 2022, Vol. 43 ›› Issue (13): 80-88.doi: 10.7506/spkx1002-6630-20210517-191

• 营养卫生 • 上一篇

微量元素硼对大鼠回肠显微结构、消化酶活性、屏障功能及抗氧化功能的影响

韩玉皎，邓娟，赵春芳，胡倩倩，任曼，顾有方，靳二辉，李升和

（1. 安徽科技学院动物科学学院，安徽凤阳 233100； 2.动物营养调控与健康安徽省重点实验室，安徽凤阳 233100）

发布日期:2022-07-27
基金资助:
国家自然科学基金青年科学基金项目（32002160）；国家自然科学基金面上项目（31672502；32172816）；安徽省高校优秀青年人才基金项目（gxyqZD2019061）；安徽省自然科学基金面上项目（2108085MC117）；安徽省协同创新项目（GXXT-2019-035）；滁州市科技计划项目（2019ZN003）；国家大学生创新创业培训计划项目（202010879042）

Effect of Boron on the Microstructure, Digestive Enzyme Activity, Barrier Function and Antioxidant Function of Rat Ileum

HAN Yujiao, DENG Juan, ZHAO Chunfang, HU Qianqian, REN Man, GU Youfang, JIN Erhui, LI Shenghe

(1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China; 2. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang 233100, China)

Published:2022-07-27

摘要/Abstract

摘要： 本实验旨在研究饮水中添加不同剂量硼元素对大鼠回肠显微结构、消化酶活性、屏障功能及抗氧化功能的影响。选用100 只刚断乳的清洁级SD大鼠，适应性饲养1 周后随机分成10 组（n＝10），对照组饮用蒸馏水，实验I～IX组分别饮用含5、10、20、40、80、160、320、480 mg/L和640 mg/L硼的蒸馏水，实验期60 d。结果表明，与对照组相比，饮水中添加5 mg/L硼的大鼠回肠绒毛高度和杯状细胞（goblet cell，GC）数量极显著增加（P＜0.01），紧密连接蛋白ZO-1（P＜0.05）和Occludin（P＜0.01）表达量显著升高，麦芽糖酶（P＜0.05）、乳糖酶（P＜0.01）、α-淀粉酶（P＜0.01）、脂肪酶（P＜0.01）活力以及总抗氧化能力（total antioxidant capacity，T-AOC）（P＜0.05）、超氧化物歧化酶（superoxide dismutase，SOD）活力（P＜0.01）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）含量（P＜0.01）显著增加。饮水添加10 mg/L硼的大鼠回肠GC（P＜0.05）和上皮内淋巴细胞（intra-epithelial lymphocytes，IEL）（P＜0.01）数量显著增加，分泌型免疫球蛋白A（secretory immunoglobulin A，SIg A）（P＜0.05）、ZO-1（P＜0.01）和Occludin（P＜0.01）表达量显著升高，乳糖酶（P＜0.01）、SOD（P＜0.01）活力和GSH-Px含量（P＜0.05）也显著增加。而饮水添加480 mg/L和640 mg/L硼的大鼠GC（P＜0.01）和IEL（P＜0.01）数量显著降低，SIg A（P＜0.01）、ZO-1（P＜0.05）和Occludin（P＜0.01）表达量均显著降低，麦芽糖酶活力以及T-AOC也显著降低（P＜0.05）。结论：饮水补充5 mg/L和10 mg/L硼可改善大鼠回肠的显微结构，增强消化酶活性、屏障功能以及抗氧化功能，而饮水补充480 mg/L和640 mg/L硼则产生相反作用。

关键词: 硼；回肠；显微结构；消化酶活性；屏障功能；抗氧化功能

Abstract: The purpose of this experiment was to study the effects of adding different doses of boron in drinking water on the microstructure, digestive enzyme activity, barrier function and antioxidant function of the rat ileum. One hundred freshly weaned clean-grade SD rats were randomly selected and divided into 10 groups (n =10) after one week of acclimatization. The control group was given distilled water, while the experimental groups I to IX were given distilled water containing 5, 10, 20, 40, 80, 160, 320, 480 and 640 mg/L of boron, respectively, for 60 days. The results showed that compared with the control group, the height of ileum villi and the number of goblet cells (GC) in rats given 5 mg/L of boron increased significantly (P < 0.01), the expression of the tight junction proteins ZO-1 (P < 0.05) and occludin (P < 0.01) increased significantly, and the activities of maltase (P < 0.05), lactase (P < 0.01), α-amylase (P < 0.01), lipase (P < 0.01), and superoxide dismutase (SOD) (P < 0.01), total antioxidant capacity (T-AOC) (P < 0.05) and the content of glutathione peroxidase (GSH-Px) (P < 0.01) also increased significantly. The numbers of ileal GC (P < 0.05) and intra-epithelial lymphocytes (IEL) (P < 0.01) increased significantly, the expression of secretory immunoglobulin A (SIg A) (P < 0.05), ZO-1 (P < 0.01) and occludin (P < 0.01) increased significantly, and the activities of lactase (P < 0.01), and SOD (P < 0.01) and GSH-Px content (P < 0.05) also increased significantly in the ileum of rats given 10 mg/L of boron in drinking water. In contrast, the number of GC (P < 0.01) and the number of IEL (P < 0.01) significantly decreased, SIg A (P < 0.01), ZO-1 (P < 0.05) and occludin (P < 0.01) expression significantly decreased, and the activity of maltase (P < 0.05) and T-AOC (P < 0.05) also significantly decreased in rats administered with 480 and 640 mg/L of boron in drinking water. These results suggest that drinking water supplementation with 5 and 10 mg/L of boron could improve the microstructure, enhance digestive enzyme activity, barrier function and antioxidant function of the rat ileum, whereas drinking water supplementation with 480 and 640 mg/L of boron could have the opposite effects.

Key words: boron; ileum; microstructure; digestive enzyme activity; barrier function; antioxidant function

中图分类号:

TS201.4

韩玉皎，邓娟，赵春芳，胡倩倩，任曼，顾有方，靳二辉，李升和. 微量元素硼对大鼠回肠显微结构、消化酶活性、屏障功能及抗氧化功能的影响[J]. 食品科学, 2022, 43(13): 80-88.

HAN Yujiao, DENG Juan, ZHAO Chunfang, HU Qianqian, REN Man, GU Youfang, JIN Erhui, LI Shenghe. Effect of Boron on the Microstructure, Digestive Enzyme Activity, Barrier Function and Antioxidant Function of Rat Ileum[J]. FOOD SCIENCE, 2022, 43(13): 80-88.

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微量元素硼对大鼠回肠显微结构、消化酶活性、屏障功能及抗氧化功能的影响

Effect of Boron on the Microstructure, Digestive Enzyme Activity, Barrier Function and Antioxidant Function of Rat Ileum

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