食品科学 ›› 2022, Vol. 43 ›› Issue (19): 151-157.doi: 10.7506/spkx1002-6630-20211006-038

• 营养卫生 • 上一篇    下一篇

磷脂型二十二碳六烯酸和二十碳五烯酸对脂多糖所致小鼠急性肝损伤的保护作用及机制

黄玉洁，郝仪铭，周梦晴，伍梓健，杨玉红，刘小芳，王保珍，杜磊   

  1. （1.山东大学齐鲁医学院公共卫生学院，山东 济南 250012；2.山东大学附属济南市中心医院专科转化研究中心，山东 济南 250013；3.齐鲁工业大学（山东省科学院）食品科学与工程学院，山东 济南 250353；4.中国水产科学研究院黄海水产研究所，农业农村部极地渔业开发重点实验室，山东 青岛 266071）
  • 出版日期:2022-10-15 发布日期:2022-10-26
  • 基金资助:
    国家自然科学基金青年科学基金项目（82003447）；山东大学青年学者未来计划项目（2018WLJH34）； 青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室开放基金项目（LMDBKF-2019-05）

Protective Effect and Mechanism of Docosahexaenoic Acid-Enriched Phospholipids and Eicosapentaenoic Acid-Enriched Phospholipids on Lipopolysaccharide-Induced Acute Liver Injury in Mice

HUANG Yujie, HAO Yiming, ZHOU Mengqing, WU Zijian, YANG Yuhong, LIU Xiaofang, WANG Baozhen, DU Lei   

  1. (1. School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, China; 2. Research Center of Translational Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, China; 3. School of Food Science & Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China; 4. Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China)
  • Online:2022-10-15 Published:2022-10-26

RichHTML

21

PDF (PC)

100

摘要： 目的：探讨磷脂型二十二碳六烯酸（docosahexaenoic acid-enriched phospholipids，DHA-PL）和磷脂型二十碳五烯酸（eicosapentaenoic acid-enriched phospholipids，EPA-PL）对脂多糖（lipopolysaccharide，LPS）所致小鼠急性肝损伤的保护作用及其机制。方法：以南海鸢乌贼（Sthenoteuthis oualaniensis）卵和冰岛刺参（Cucumaria frondosa）体壁为原料，分离提取DHA-PL和EPA-PL。将C57BL/6J雄性小鼠随机分为Control组、模型组、LPS＋DHA-PL组和LPS＋EPA-PL组，Control组和模型组小鼠每天灌胃20 mL/kg mb生理盐水，其他组以400 mg/kg mb剂量分别每天灌胃受试物，灌胃体积为10 mL/kg mb，连续干预28 d；第29天Control组腹腔注射无菌生理盐水，其他组注射LPS（10 mg/kg mb），注射体积为10 mL/kg mb，建立急性肝损伤模型；称量小鼠体质量和肝质量，计算肝指数；测定血清中丙氨酸氨基转移酶（alanine aminotransferase，ALT）和天冬氨酸氨基转移酶（aspartate aminotransferase，AST）活力；苏木精-伊红染色观察肝组织病理改变情况；采用实时荧光定量聚合酶链式反应和酶联免疫吸附试剂盒测定肝脏中肿瘤坏死因子（tumor necrosis factor，TNF）-α、白细胞介素（interleukin，IL）-1β和IL-6的mRNA相对表达量和含量；采用蛋白免疫印迹法考察肝脏中丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）如细胞外调节蛋白激酶（extracellular regulated protein kinases 1/2，ERK1/2）、c-Jun氨基末端激酶（c-Jun N-terminal kinase，JNK）、p38以及核因子（nuclear factor，NF）-κB p65蛋白磷酸化水平。结果：DHA-PL和EPA-PL预防性干预能有效保护LPS所致小鼠急性肝损伤，降低肝指数以及血清ALT和AST活力，下调肝脏中TNF-α、IL-1β和IL-6含量，降低MAPK和NF-κB p65蛋白磷酸化水平。结论：DHA-PL和EPA-PL预防性干预可保护LPS所致小鼠急性肝损伤，其作用机制可能依赖于其对MAPK和NF-κB信号通路的调控。

关键词: 磷脂型二十二碳六烯酸；磷脂型二十碳五烯酸；急性肝损伤；丝裂原活化蛋白激酶；核因子-κB

Abstract: Purpose: To investigate the protective effect and mechanism of docosahexaenoic acid-enriched phospholipids (DHA-PL) and eicosapentaenoic acid-enriched phospholipids (EPA-PL) on lipopolysaccharide (LPS)-induced acute liver injury. Methods: DHA-PL and DHA-PL were obtained from the eggs of Sthenoteuthis oualaniensis and the body wall of Cucumaria frondosa, respectively. C57BL/6 male mice were randomly divided into four groups, i.e. control group, LPS-induced model group, LPS + DHA-PL group and LPS + EPA-PL group. The mice in the LPS + DHA-PL and LPS + EPA-PL groups were given DHA-PL and EPA-PL via the intragastric route at 400 mg/kg mb on a daily basis for 28 days, respectively, while those in the control and model groups were gavaged with normal saline. The mice in the control group were injected with normal saline intraperitoneally at day 29, while those in the other groups were injected with LPS (10 mg/kg mb) at an injection volume of 10 mL/kg mb to establish a model of acute liver injury. The pathological changes of liver tissues were observed by hematoxylin-eosin (HE) staining. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured. The transcriptional levels and contents of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in liver tissues were detected by real-time quantitative polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA), respectively. The phosphorylation levels of mitogen-activated protein kinases (MAPKs) such as extracellular regulated protein kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38, and nuclear factor (NF)-κB p65 were determined by Western blotting. Results: DHA-PL and EPA-PL intervention effectively alleviated LPS-induced acute liver injury and reduced hepatic index and serum ALT and AST activities, decreased the hepatic mRNA expression and contents of TNF-α, IL-1β and IL-6, and down-regulated the phosphorylation of ERK1/2, JNK, p38 and NF-κB p65. Conclusion: DHA-PL and EPA-PL can prevent LPS-induced acute liver injury in mice, and the mechanism may depend on regulation of the MAPKs and NF-κB signaling pathways.

Key words: docosahexaenoic acid-enriched phospholipids; eicosapentaenoic acid-enriched phospholipids; acute liver injury; mitogen-activated protein kinase; nuclear factor κB

中图分类号: 

京ICP备14027880号-3　京公网安备 11010202009729号
地址：北京市丰台区洋桥70号 中国食品杂志社《食品科学》编辑部　邮编：100068
电话：010-83155446/47/48/49/50　 传真：010-83155436　 Email：foodsci@126.com
本系统由北京玛格泰克科技发展有限公司设计开发　技术支持：support@magtech.com.cn