植物乳杆菌KSFY04通过调节核因子κB信号通路干预小鼠血栓形成的效果

doi:10.7506/spkx1002-6630-20211107-076

食品科学 ›› 2022, Vol. 43 ›› Issue (23): 158-167.doi: 10.7506/spkx1002-6630-20211107-076

植物乳杆菌KSFY04通过调节核因子κB信号通路干预小鼠血栓形成的效果

赵欣，刘佳，易若琨，骞宇，杨贞耐

（1.重庆第二师范学院重庆市功能性食品协同创新中心，重庆 400067；2.北京工商大学北京食品营养与人类健康高精尖创新中心，北京 100048）

出版日期:2022-12-15 发布日期:2022-12-28
基金资助:
重庆市高校创新研究群体项目（CXQTP20033）；重庆市教育委员会科学技术研究项目重大项目（KJZD-M202201601）；重庆市自然科学基金面上项目（CSTB2022NSCQ-MSX0848）；重庆市留学人员回国创业创新支持计划项目（2205012980094776）

Lactobacillus plantarum KSFY04 Inhibits Thrombosis in Mice by Regulating the Nuclear Factor Kappa B (NF-κB) Signaling Pathway

ZHAO Xin, LIU Jia, YI Ruokun, QIAN Yu, YANG Zhennai

(1. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China; 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, China)

Online:2022-12-15 Published:2022-12-28

摘要/Abstract

摘要： 通过角叉菜胶建立小鼠血栓模型，观察植物乳杆菌KSFY04（Lactobacillus plantarum KSFY04，LP-KSFY04）通过调节核因子κB（nuclear factor κB，NF-κB）相关炎症通路抑制血栓形成的效果。采用生化试剂盒和定量聚合酶链式反应（quantitative polymerase chain reaction，qPCR）检测小鼠血清、组织相关指标，苏木精-伊红（hematoxylin-eosin，HE）切片观察组织病变，并观察肠道微生物组成。结果：LP-KSFY04能够有效减轻血栓小鼠黑尾程度，并延长血栓小鼠活化部分凝血活酶时间、缩短凝血酶原时间、凝血酶时间及降低纤维蛋白原水平。LP-KSFY04还能够降低血栓小鼠血清、肾脏和肝脏炎症细胞因子肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、白细胞介素（interleukin，IL）6和IL-1β的水平。病理学观察发现LP-KSFY04能够减轻血栓造成的肝肾组织病变和尾静脉血栓积累。qPCR结果显示，LP-KSFY04下调血栓小鼠尾静脉血管组织NF-κB p65、IL-6、TNF-α、细胞间黏附分子1（intercellular cell adhesion molecule 1，ICAM-1）、血管细胞黏附分子1（vascular cell adhesion molecule 1，VCAM-1）和选择素E的mRNA表达水平。同时，LP-KSFY04能够增加拟杆菌门、乳酸杆菌属、双歧杆菌属的丰度并降低厚壁菌门丰度。结论：LP-KSFY04对血栓小鼠具有减轻炎症和抑制血栓形成的作用，且高剂量LP-KSFY04作用更好，效果接近药物双嘧达莫。

关键词: 血栓；植物乳杆菌；核因子κB信号通路；凝血；免疫

Abstract: This study observed the effect of Lactobacillus plantarum KSFY04 (LP-KSFY04) against carrageenan-induced thrombosis in mice by regulating the NF-κB signaling pathway. Commercial biochemical kits and quantitative polymerase chain reaction (qPCR) were used to detect related indexes in mouse serum and tissues, and hematoxylin-eosin (HE) staining was used for histopathological examination. The composition of intestinal microbiota was examined as well. The results showed that LP-KSFY04 could effectively reduce the degree of black tail in thrombotic mice, prolong the activated partial thromboplastin time (APTT), and reduce the prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) level. LP-KSFY04 could also reduce the levels of inflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) in the serum, kidney and liver of thrombotic mice. Pathological observation showed that LP-KSFY04 could reduce liver and kidney lesions caused by thrombosis and the accumulation of tail vein thrombosis. qPCR results showed that LP-KSFY04 could down-regulate the mRNA expression of nuclear factor kappa-B p65 (NF-κB p65), IL-6, TNF-α, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin. Moreover, LP-KSFY04 increased the relative abundance of Bacteroidetes, Lactobacillus and Bifidobacterium and decreased the relative abundance of Firmicutes. These results showed that LP-KSFY04 can reduce inflammation and inhibit thrombosis in thrombotic mice, and the effects of high concentration of LP-KSFY04 were most pronounced, which were close to those of dipyridamole.

Key words: thrombus; Lactobacillus plantarum; nuclear factor κB signaling pathway; coagulation; immune

中图分类号:

TS218

赵欣，刘佳，易若琨，骞宇，杨贞耐. 植物乳杆菌KSFY04通过调节核因子κB信号通路干预小鼠血栓形成的效果[J]. 食品科学, 2022, 43(23): 158-167.

ZHAO Xin, LIU Jia, YI Ruokun, QIAN Yu, YANG Zhennai. Lactobacillus plantarum KSFY04 Inhibits Thrombosis in Mice by Regulating the Nuclear Factor Kappa B (NF-κB) Signaling Pathway[J]. FOOD SCIENCE, 2022, 43(23): 158-167.

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植物乳杆菌KSFY04通过调节核因子κB信号通路干预小鼠血栓形成的效果

Lactobacillus plantarum KSFY04 Inhibits Thrombosis in Mice by Regulating the Nuclear Factor Kappa B (NF-κB) Signaling Pathway

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