基于LAMP技术快速检测冷鲜鸡新鲜度

doi:10.7506/spkx1002-6630-20220304-060

食品科学 ›› 2023, Vol. 44 ›› Issue (6): 351-359.doi: 10.7506/spkx1002-6630-20220304-060

基于LAMP技术快速检测冷鲜鸡新鲜度

张晋豪，王浩东，刘欣悦，陈珊珊，胡海静，徐幸莲，王虎虎

（南京农业大学肉品加工与质量控制教育部重点实验室，江苏南京 210095）

出版日期:2023-03-27 发布日期:2023-03-27
基金资助:
国家自然科学基金面上项目（32172266）；江苏省自然科学基金面上项目（BK20221515）

Loop-Mediated Isothermal Amplification for Rapid Evaluation of the Freshness of Chilled Chicken

ZHANG Jinhao, WANG Haodong, LIU Xinyue, CHEN Shanshan, HU Haijing, XU Xinglian, WANG Huhu

(Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China)

Online:2023-03-27 Published:2023-03-27

摘要/Abstract

摘要： 依据冷鲜鸡新鲜度与假单胞菌数量的高度正相关性为基础，建立一种利用环介导等温扩增（loop-mediated isothermal amplification，LAMP）技术快速检测假单胞菌数量从而实现新鲜度检测的技术。结果显示：建立的LAMP体系对假单胞菌特异性扩增极高，灵敏度最高达到3.05×102 CFU/mL；根据贮藏期间冷鲜鸡中假单胞菌数、菌落总数、挥发性盐基氮、pH值和感官属性等指标测定结果，发现通过菌落总数和气味属性指示新鲜度最准确，而假单胞菌数与二者相关性最强；通过LAMP技术能准确判别腐败初期的假单胞菌数，进而推测冷鲜鸡的新鲜度。本研究建立的方法省时、灵活，在多种场景下均具有实用性，有一定的推广价值。

关键词: 冷鲜鸡新鲜度；假单胞菌属；环介导等温扩增技术

Abstract: Based on the highly positive correlation between chilled chicken freshness and the number of Pseudomonas, this study established a loop-mediated isothermal amplification (LAMP) method for rapid detection of Pseudomonas for the purpose of freshness detection. The results showed that the established LAMP system had high specificity for Pseudomonas, and the highest sensitivity was 3.05 × 102 CFU/mL. According to the measured values of Pseudomonas count, total bacterial count, total volatile basic nitrogen (TVB-N) content, pH, sensory properties of chilled chicken during storage, it was found that the total bacterial count and odor properties were the most accurate indicators of freshness, and the correlation between Pseudomonas count and them was the strongest. Pseudomonas count in the early stage of spoilage could be accurately identified by LAMP, and based on it the freshness of chilled chicken could be inferred. The method established in this study is time-saving, flexible, and applicable to a variety of scenarios, and it deserves to be popularized.

Key words: freshness of chilled chicken; Pseudomonas; loop-mediated isothermal amplification

中图分类号:

TS251.7

张晋豪，王浩东，刘欣悦，陈珊珊，胡海静，徐幸莲，王虎虎. 基于LAMP技术快速检测冷鲜鸡新鲜度[J]. 食品科学, 2023, 44(6): 351-359.

ZHANG Jinhao, WANG Haodong, LIU Xinyue, CHEN Shanshan, HU Haijing, XU Xinglian, WANG Huhu. Loop-Mediated Isothermal Amplification for Rapid Evaluation of the Freshness of Chilled Chicken[J]. FOOD SCIENCE, 2023, 44(6): 351-359.

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基于LAMP技术快速检测冷鲜鸡新鲜度

Loop-Mediated Isothermal Amplification for Rapid Evaluation of the Freshness of Chilled Chicken

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