食品科学 ›› 2022, Vol. 43 ›› Issue (8): 339-345.doi: 10.7506/spkx1002-6630-20210510-107

• 安全检测 • 上一篇    

基于镍基碱式碳酸盐修饰电极的无酶葡萄糖电化学传感器构建

刘丽,张智锋,王松磊,李海峰,李恳恳,马亮,魏培媛,张也,武康宁   

  1. (1.宁夏大学食品与葡萄酒学院,宁夏 银川 750021;2.华中农业大学食品科学技术学院,湖北 武汉 430000;3.宁夏华信达健康科技有限公司,宁夏 灵武 751400)
  • 发布日期:2022-04-26
  • 基金资助:
    宁夏回族自治区重点研发计划项目(2019BEH03002);国家自然科学基金地区科学基金项目(31660484)

Enzyme-free Electrochemical Sensor for Glucose Detection Based on Nickel-based Basic Carbonate Modified Electrode

LIU Li, ZHANG Zhifeng, WANG Songlei, LI Haifeng, LI Kenken, MA Liang, WEI Peiyuan, ZHANG Ye, WU Kangning   

  1. (1. School of Food and Wine, Ningxia University, Yinchuan 750021, China; 2. School of Food Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; 3. Huaxinda Health Technology Company of Ningxia, Lingwu 751400, China)
  • Published:2022-04-26

摘要: 在碳纸基底上采用一步水热法原位生长过渡金属镍基碱式碳酸盐(Ni-CHs),构建高灵敏度和选择性的无酶葡萄糖传感器。利用X射线衍射、扫描电子显微镜对材料进行物相鉴定和形貌表征。采用循环伏安法和时间-电流曲线评估传感器的性能。结果表明,Ni-CHs/CP在0.5 V的低电势下对葡萄糖具有最佳检测能力,传感器的氧化峰电流在葡萄糖浓度为0.95 μmol/L~2.623 mmol/L范围内呈现良好的线性关系,相关系数为0.998,检出限为0.31 μmol/L(RSN=3),响应时间为3.5 s。此传感器具有良好的稳定性并成功用于实际样品的检测,加标回收率在95.65%~105.56%之间。

关键词: 镍基碱式碳酸盐;无酶葡萄糖传感器;水热法;饮料

Abstract: Based on transition metal nickel-based basic carbonate (Ni-CHs) grown in situ on carbon paper (Ni-CHs/CP), prepared by one-step hydrothermal method, a highly sensitive and selective enzyme-free sensor to detect glucose was developed. X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used to identify the phase and characterize the morphology of the material. Cyclic voltammetry (CV) and time-current curve were used to evaluate the performance of the sensor. The results showed that Ni-CHs/CP had the best detection ability for glucose at a low potential of 0.5 V, and the oxidation peak current of the sensor showed an excellent linear relationship with glucose concentration in the range of 0.95 μmol/L–2.623 mmol/L, with a correlation coefficient of 0.998. The detection limit was 0.31 μmol/L (RSN = 3) and response time was 3.5 s. This sensor has good stability and has been successfully used in the detection of actual samples with spiked recoveries of 95.65%–105.56%.

Key words: nickel-base carbonate; non?enzymatic electrochemical sensor for glucose detection; hydrothermal method; beverage

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