食品科学 ›› 2023, Vol. 44 ›› Issue (20): 252-259.doi: 10.7506/spkx1002-6630-20221127-309

• 成分分析 • 上一篇    下一篇

植物乳杆菌发酵人参中活性成分改变及抗氧化作用

刘士伟,刘胜楠,米倩雯,阴裴,薛婷芳,于晓然,孟星坚,王丽娜,毕云枫   

  1. (1.吉林农业大学食品科学与工程学院,吉林 长春 130118;2.吉林医药学院药学院,吉林 吉林 132013)
  • 出版日期:2023-10-25 发布日期:2023-11-07
  • 基金资助:
    吉林省科技厅重点研发项目(20210204179YY)

Changes in Active Components and Antioxidant Properties of Ginseng Fermented by Lactobacillus plantarum

LIU Shiwei, LIU Shengnan, MI Qianwen, YIN Pei, XUE Tingfang, YU Xiaoran, MENG Xingjian, WANG Lina, BI Yunfeng   

  1. (1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China;2. College of Pharmacy, Jilin Medical University, Jilin 132013, China)
  • Online:2023-10-25 Published:2023-11-07

摘要: 通过益生菌植物乳杆菌对人参提取物进行发酵,探究发酵前后活性成分改变情况及其抗氧化活性。测定发酵前后人参提取物中总酚、多糖、总黄酮和人参皂苷的含量,并通过测定3 种自由基清除率、还原力及脂质抗氧化能力等抗氧化活性指标,比较发酵前后人参提取物的抗氧化能力。结果表明,发酵后人参提取物主要活性物质总酚、总黄酮的含量提高,多糖和总皂苷的含量减少,发酵过程中天然人参皂苷Rg1、Re、Rb1和Rc上的糖基经过水解作用,生成新的稀有人参皂苷Ck、Rk1、Rh4、Rg5等,其含量分别增加了0.770 6、0.734 8、3.392 4、1.364 8 mg/g。发酵后人参提取物在质量浓度为0.5~20.0 mg/mL的范围内抗氧化能力比发酵前高,这与总酚、总黄酮含量增加及稀有人参皂苷生成有密不可分的关系。这说明发酵能够生成大量稀有皂苷,同时增加酚类和黄酮类化合物的含量,使发酵人参提取物具有更强的抗氧化活性。

关键词: 人参提取物;发酵;植物乳杆菌;抗氧化

Abstract: Ginseng extract was fermented by the probiotic Lactobacillus plantarum to investigate the changes in its active ingredients and antioxidant activities before and after fermentation. The contents of total phenols, polysaccharides, total flavonoids and ginsenosides in raw and fermented ginseng extract were determined, and their antioxidant activities were evaluated by determining three free radical scavenging capacities, reducing power and lipid oxidation inhibitory activity. The results showed that the contents of total phenols, total flavonoids increased, while the contents of polysaccharides and total saponins decreased after fermentation. Ginsenosides Rg1, Re, Rb1 and Rc were hydrolyzed into rare ginsenosides such as Ck, Rk1, Rh4, and Rg5 during the fermentation process, and their contents increased by 0.770 6, 0.734 8, 3.392 4 and 1.364 8 mg/g, respectively. The antioxidant capacity of fermented ginseng extract at concentrations of 0.5–20 mg/mL increased compared with that before fermentation, which was inextricably related to the increase in the contents of total phenols and total flavonoids and the generation of rare ginsenosides. This shows that the fermentation process can produce a large amount of rare saponins and increase the contents of phenols and flavonoids, which makes fermented ginseng extract have stronger antioxidant ability.

Key words: ginseng extract; fermentation; Lactobacillus plantarum; antioxidant

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