食品科学 ›› 2024, Vol. 45 ›› Issue (4): 225-231.doi: 10.7506/spkx1002-6630-20230429-286

• 成分分析 • 上一篇    下一篇

基于感官组学分析鉴定无花果提取物关键特征风味物质

王新惠,何峰,李瑞丽,王翰书,梁淼,张逸寒,潘文亮,张峻松   

  1. (1.郑州轻工业大学食品与生物工程学院,河南 郑州 450000;2.河北中烟工业有限责任公司技术中心,河北 石家庄 050051)
  • 出版日期:2024-02-25 发布日期:2024-03-11
  • 基金资助:
    河北中烟工业有限责任公司重点项目(HBZY2023A039)

Identification of Key Aroma Compounds in Fig Extract through Sensomics Approach

WANG Xinhui, HE Feng, LI Ruili, WANG Hanshu, LIANG Miao, ZHANG Yihan, PAN Wenliang, ZHANG Junsong   

  1. (1. School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450000, China; 2. Technical Center, Hebei Tobacco Industry Co. Ltd., Shijiazhuang 050051, China)
  • Online:2024-02-25 Published:2024-03-11

摘要: 采用顶空固相微萃取-气相色谱-质谱(headspace solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)联用技术、气相色谱-嗅闻(gas chromatography-olfactometry,GC-O)技术结合香气活性值(odor activity value,OAV)及香气重组与缺失实验确定无花果提取物的关键特征风味成分。结果表明,无花果提取物经HS-SPME-GC-MS与GC-O共鉴定出31 种化合物,其中异丁酸、γ-丁内酯、芳樟醇、壬醛、香兰素、糠醛等18 种化合物为无花果提取物重要香气成分(OAV>1);重组实验表明重组样品与无花果提取物的感官属性评价结果接近,表现为果香、甜香、烘焙香、焦糖香等典型的风味特征,略带酸香、膏香、奶香,具有明显的无花果提取物风味特征;缺失实验进一步明确了γ-己内酯、棕榈酸乙酯、苯甲醇、芳樟醇、香兰素、苯甲醛、4-羟基-2,5-二甲基-3(2H)-呋喃酮、5-羟甲基糠醛、甲基环戊烯醇酮为无花果提取物关键特征风味物质。该研究结果可为无花果特征风味香原料的开发及品质控制提供理论基础。

关键词: 无花果提取物;气相色谱-质谱;气相色谱-嗅闻;香气重组与缺失实验;关键特征风味物质

Abstract: In this study, headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and gas chromatography-olfactory (GC-O) were applied to identify and analyze the volatile aroma compounds of the ethanol extract of figs. Its key characteristic flavor compounds were analyzed by odor activity (OAV) and aroma recombination and omission tests. The results showed that a total of 40 volatile aroma components were identified, of which 18 compounds, such as isobutyrate, γ-butyl lactone, aromatic camphor, nonaldehyde, vanillin and furfural, were important aroma components in the fig extract (OAV > 1). The aroma recombination experiments showed that the sensory properties of the recombined samples, which had typical aroma characteristics such as fruity, sweet, baked and burnt sweet with slight sour, ointment-like and milky, were similar to those of the fig extract. Furthermore, the aroma omission experiments identified gamma-hexanoate, ethyl palmitate, phenmethanol, aromatic camphor, vanillin, benzaldehyde, 4-hydroxy-2,5-dimethyl-3 (2H)-furan, 5-hydroxymyfuran, and methyl cyclopentanolone as key characteristic flavor compounds of the fig extract. The findings of this study provide a theoretical basis for the development and quality control of fig characteristic flavorings.

Key words: fig extract; gas chromatography-mass spectrometry; gas chromatography-olfactometry; aroma recombination and omission experiment; key characteristic flavor substances

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