茉莉酸甲酯类生长调节剂对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径的影响

doi:10.7506/spkx1002-6630-20230602-009

食品科学 ›› 2024, Vol. 45 ›› Issue (8): 248-256.doi: 10.7506/spkx1002-6630-20230602-009

茉莉酸甲酯类生长调节剂对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径的影响

冶楠，张珍，吕转转，杜建明，张生祥，李蔚

（1.甘肃农业大学食品科学与工程学院，甘肃兰州 730070；2.甘肃省葡萄与葡萄酒工程学重点实验室，甘肃省葡萄与葡萄酒产业技术研发中心，甘肃兰州 730070）

出版日期:2024-04-25 发布日期:2024-05-01
基金资助:
甘肃农业大学公招博士科研启动基金项目（GAU-KYQD-2020-21）；甘肃省青年科技基金计划项目（21JR7RA849）

Effects of the Plant Growth Regulator Methyl Jasmonate on Fruit Quality and Lipoxygenase Metabolic Pathway of Cabernet Gernischt

YE Nan, ZHANG Zhen, LÜ Zhuanzhuan, DU Jianming, ZHANG Shengxiang, LI Wei

(1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. Gansu Key Laboratory of Viticulture and Enology, Research and Development Center of Wine Industry in Gansu Province, Lanzhou 730070, China)

Online:2024-04-25 Published:2024-05-01

摘要/Abstract

摘要： 以‘蛇龙珠’葡萄果实为试材，通过测定其基本理化指标，筛选茉莉酸甲酯（methyl jasmonate，MeJA）类物质最适浓度，同时结合顶空固相微萃取-气相色谱-质谱联用技术及酶联免疫吸附试验分析MeJA类物质响应的挥发性关键代谢物及关键酶活性，探究采前MeJA类物质处理对‘蛇龙珠’葡萄果实品质及脂氧合酶（lipoxygenase，LOX）代谢途径中绿叶挥发物的影响。结果表明，与对照组相比，0.1 mmol/L MeJA和5 mmol/L二氢茉莉酸甲酯处理可显著提高‘蛇龙珠’葡萄果实百粒质量、总糖质量浓度、可溶性固形物质量分数、pH值、总酚及总花色苷含量，在采收期时分别提高了10.24%、27.83%、28.24%、4.80%、77.63%、85.29%和9.15%、24.01%、24.41%、8.30%、69.21%、71.57%，果实可滴定酸质量浓度分别降低40.0%及30.24%，果实品质得到显著提高和改善。此浓度处理后，‘蛇龙珠’葡萄果实C6类化合物含量明显提高，更加突出其果实香气的典型性。同时，LOX代谢途径中各个关键酶活性与代谢产物之间具有显著相关性，说明较适浓度的MeJA类物质处理对‘蛇龙珠’葡萄果实中绿叶挥发物的积累具有积极作用。

关键词: ‘蛇龙珠’；茉莉酸甲酯；二氢茉莉酸甲酯；葡萄品质；脂氧合酶代谢

Abstract: In this study, the optimal concentrations of methyl jasmonate (MeJA) and methyl dihydrojasmonate (MDJA) for Cabernet Gernischt were selected based on basic physicochemical properties of grapes. The key volatile metabolites and key enzyme activities in response to MeJA and MDJA were analyzed by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and enzyme-linked immunosorbent assay (ELISA), respectively. The effects of pre-harvest MeJA or MDJA treatment on the fruit quality and green leaf volatile components in the lipoxygenase (LOX) metabolic pathway of Cabernet Gernischt were also investigated. The findings revealed that the application of 0.1 mmol/L MeJA or 5 mmol/L MDJA significantly enhanced mass of 100 berries, total sugar content, soluble solids content, pH, total phenol and total anthocyanin contents. Compared with the control group, MeJA increased these indexes by 10.24%, 27.83%, 28.24%, 4.80%, 77.63% and 85.29%, respectively, while MDJA increased them by 9.15%, 24.01%, 24.41%, 8.30%, 69.21% and 71.57%, respectively. Furthermore, MeJA and MDJA reduced titratable acid content by 40.0% and 30.24%, respectively. Accordingly, both MeJA and MDJA significantly improved fruit quality. Moreover, they significantly increased the contents of C6 compounds responsible for the typical aroma of Cabernet Gernischet. Significant correlations were observed between the activity of each key enzyme in the lipoxygenase metabolic pathway and the corresponding metabolites, indicating that the application of MeJA or MDJA at a suitable concentration could positively influence the accumulation of green leaf volatiles in Cabernet Gernischt.

Key words: Cabernet Gernischt; methyl jasmonate; methyl dihydrojasmonate; grape quality; lipoxygenase metabolism

中图分类号:

S663.1

冶楠，张珍，吕转转，杜建明，张生祥，李蔚. 茉莉酸甲酯类生长调节剂对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径的影响[J]. 食品科学, 2024, 45(8): 248-256.

YE Nan, ZHANG Zhen, LÜ Zhuanzhuan, DU Jianming, ZHANG Shengxiang, LI Wei. Effects of the Plant Growth Regulator Methyl Jasmonate on Fruit Quality and Lipoxygenase Metabolic Pathway of Cabernet Gernischt[J]. FOOD SCIENCE, 2024, 45(8): 248-256.

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茉莉酸甲酯类生长调节剂对‘蛇龙珠’葡萄果实品质及脂氧合酶代谢途径的影响

Effects of the Plant Growth Regulator Methyl Jasmonate on Fruit Quality and Lipoxygenase Metabolic Pathway of Cabernet Gernischt

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