不同禁食模式联合运动训练对小鼠白色脂肪棕色化的影响

doi:10.7506/spkx1002-6630-20240213-072

食品科学 ›› 2024, Vol. 45 ›› Issue (20): 169-179.doi: 10.7506/spkx1002-6630-20240213-072

不同禁食模式联合运动训练对小鼠白色脂肪棕色化的影响

李银焕, 王旭锴, 史健, 徐林菁, 周李姿, 李伟业, 刘辉, 柳春红

（1.华南农业大学食品学院，广东省食品质量安全重点实验室，广东广州 510642；2.广东产品质量监督检验研究院，广东广州 510670）

出版日期:2024-10-25 发布日期:2024-10-14
基金资助:
“十四五”国家重点研发计划重点专项（2023YFF1104204）；广东省自然科学基金项目（2021A1515010825）；广东省食品质量安全重点实验室项目（2020B1212060059）

Effects of Different Fasting Modes Combined with Exercise Training on Browning of White Adipose Tissue in Mice

LI Yinhuan, WANG Xukai, SHI Jian, XU Linjing, ZHOU Lizi, LI Weiye, LIU Hui, LIU Chunhong

(1. The key Laboratory of Food Quality and Safety of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Guangdong Testing Institute of Product Quality Supervision, Guangzhou 510670, China)

Online:2024-10-25 Published:2024-10-14

摘要/Abstract

摘要： 为了探讨间歇性禁食联合运动训练对白色脂肪棕色化的影响，本实验将5～6 周龄雄性C57BL/6小鼠随机分为6 组：对照组、运动组、隔日禁食组、隔日禁食运动组、限时禁食组、限时禁食运动组。实验6 周后测定小鼠血脂水平，并通过Western blot检测小鼠双侧腹股沟脂肪、肾周脂肪、附睾周脂肪、肩胛间棕色脂肪及左侧骨骼肌样本中磷酸化腺苷酸活化蛋白激酶（phosphorylated-adenosine monophosphate activated protein kinase，p-AMPK）、过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor gamma，PPARγ）、氧化物酶体增殖物激活受体γ辅活化因子-1α（peroxisome proliferator-activated receptor gamma coactivator-1 alpha，PGC-1α）、解偶联蛋白1（uncoupling protein 1，UCP1）、特异性蛋白含III型纤连蛋白域蛋白5（active fibronectin type III domain containing protein 5，FNDC5）以及鸢尾素和PR结构域蛋白16（PR domain-containing 16，PRDM16）的表达水平，分析两种间歇性禁食模式联合运动训练对小鼠白色脂肪棕色化相关通路蛋白表达量的影响，以及不同脂肪组织中发生棕色化作用的程度。结果显示，各实验组总胆固醇水平与对照组相比均显著降低；限时禁食组和限时禁食运动组中的甘油三酯、低密度脂蛋白胆固醇水平与对照组相比均显著降低（P＜0.05）；隔日禁食组、隔日禁食运动组、限时禁食组和限时禁食运动组中的p-AMPK、PPARγ、PGC-1α、UCP1、FNDC5和PRDM16在不同脂肪组织和骨骼肌中的表达量与对照组相比均有不同程度的升高，其中限时禁食运动组中p-AMPK、PPARγ、PGC-1α和UCP1在棕色脂肪、腹股沟脂肪和肾周脂肪组织中的相对表达量与对照组相比均显著升高。上述结果表明，间歇性禁食及联合作用能够促进白色脂肪组织棕色化，其机制可能与PPARγ/PGC-1α/ UCP1通路激活有关，其中限时禁食法联合运动的作用效果相对最佳。

关键词: 间歇性禁食；运动；白色脂肪组织；棕色脂肪组织；白色脂肪棕色化

Abstract: To investigate the effect of intermittent fasting (IF) combined with exercise on browning of white adipose tissue (WAT), we randomly divided male C57BL/6 mice aged 5–6 weeks into six groups: control (Con), exercise (Ex), alternate-day fasting (ADF), alternate-day fasting combined with exercise (ADFEx), time-restricted feeding (TRF), and time-restricted feeding combined with exercise (TRFEx). After the six-week experimental period, blood lipid levels of mice were tested. Next, Western blot was used to detect the expression levels of phosphorylated adenosine monophosphate activated protein kinase (p-AMPK), peroxisome proliferator-activated receptor gamma (PPARγ), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), uncoupling protein 1 (UCP1), active fibronectin type III domain containing protein 5 (FNDC5) and PR domain-containing 16 (PRDM16) in the bilateral inguinal white adipose tissue (in-WAT), perirenal adipose tissue (pe-WAT), epididymal adipose tissue (ep-WAT), interscapular brown adipose tissue (BAT), and left hindlimb skeletal muscle of mice. The effect of IF combined with exercise on protein expression in WAT browning-related signaling pathways in mice was analyzed, and the degrees of browning in different adipose tissues were compared. The results showed that the level of total cholesterol (TC) in each experimental group was significantly lower than that of the Con group. The levels of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) in the TRF and TRFEx groups were significantly lower than those in the Con group (P < 0.05). The relative expression levels of p-AMPK, PPARγ, PGC-1α, UCP1, FNDC5 and PRDM16 in different adipose tissues and skeletal muscle increased to different extents in the ADF, ADFEx, TRF and TRFEx groups compared with the Con group; the relative expression levels of p-AMPK, PPARγ, PGC-1α and UCP1 in BAT, in-WAT and pe-WAT increased significantly in the TRFEx group compared with the Con group. These results suggested that the combination of IF and exercise promoted the browning of WAT, and the mechanism might be related to the activation of the PPARγ/PGC-1α/UCP1 signaling pathway. TRFEx was most effective among all treatments.

Key words: intermittent fasting; exercise; white adipose tissue; brown adipose tissue; browning of white adipose tissue

中图分类号:

TS201.4

李银焕, 王旭锴, 史健, 徐林菁, 周李姿, 李伟业, 刘辉, 柳春红. 不同禁食模式联合运动训练对小鼠白色脂肪棕色化的影响[J]. 食品科学, 2024, 45(20): 169-179.

LI Yinhuan, WANG Xukai, SHI Jian, XU Linjing, ZHOU Lizi, LI Weiye, LIU Hui, LIU Chunhong. Effects of Different Fasting Modes Combined with Exercise Training on Browning of White Adipose Tissue in Mice[J]. FOOD SCIENCE, 2024, 45(20): 169-179.

[1]	高铭蔚张梓文朱珍珠. 大豆外泌体样囊泡对高脂线虫模型脂质代谢的调控作用[J]. 食品科学, 2024, 45(22): 0-0.
[2]	陈峻波李延啸闫巧娟江君杨绍青江正强. 褐藻寡糖对NG-硝基-L-精氨酸甲酯诱导小鼠高血压的改善作用[J]. 食品科学, 2024, 45(22): 0-0.
[3]	刘志成胡秋辉赵立艳马高兴. 杀菌方法对猴头菇山药复合饮料品质及货架期的影响[J]. 食品科学, 0, (): 0-0.
[4]	石径田顺风马淑平姜燕飞罗永康赵春月. 胶原蛋白肽改善和延缓皮肤衰老作用功效及机制的研究进展[J]. 食品科学, 0, (): 0-0.
[5]	吴雪李毅诺彭强韩林王敏. 沙棘原花青素的抗氧化和α-葡萄糖苷酶抑制活性分析[J]. 食品科学, 0, (): 0-0.
[6]	王召君, 王兆石, 赵保民, 董明盛. 丹贝冻干粉对蛋白质营养不良小鼠的改善作用[J]. 食品科学, 2024, 45(20): 180-187.
[7]	徐豪伟, 林松毅, 刘瑶, 刘柯欣, 胡胜杰, 孙娜. 南极磷虾蛋白体外消化产物特性及其成骨活性分析[J]. 食品科学, 2024, 45(20): 188-197.
[8]	刘建辉, 戴燚, 岳玉玲, 刘拓宇, 杨文建, 陆欢. 食药用菌多糖调控胃肠道功能的研究进展[J]. 食品科学, 2024, 45(20): 270-279.
[9]	郑红星, 冀乐, 祁珊珊. 微生物-肠-肝轴与酒精性肝病及其营养干预研究进展[J]. 食品科学, 2024, 45(20): 366-375.
[10]	刘建辉戴燚岳玉玲刘拓宇杨文建陆欢. 食药用菌多糖调控胃肠道功能的研究进展[J]. 食品科学, 0, (): 0-0.
[11]	郑红星冀乐祁珊珊. 微生物-肠-肝轴与酒精性肝病及其营养干预研究进展[J]. 食品科学, 0, (): 0-0.
[12]	李银焕王旭锴史健徐林菁周李姿李伟业刘辉柳春红. 不同禁食模式联合运动训练对小鼠白色脂肪棕色化的影响[J]. 食品科学, 0, (): 0-0.
[13]	明建唐源刘梦聪刘燕李富华赵吉春. 香辛料提取物改善水产品蛋白质过氧化研究进展[J]. 食品科学, 0, (): 0-0.
[14]	王召君王兆石赵保民董明盛. 丹贝冻干粉对蛋白质营养不良小鼠的改善作用[J]. 食品科学, 0, (): 0-0.
[15]	杨萍李蓉李明鉴刘博文张镨月叶子茹李敏杨岚龚艳菊黄永. 发酵枸杞多糖对D-半乳糖致衰老模型小鼠肠道的保护作用[J]. 食品科学, 0, (): 0-0.

不同禁食模式联合运动训练对小鼠白色脂肪棕色化的影响

Effects of Different Fasting Modes Combined with Exercise Training on Browning of White Adipose Tissue in Mice

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价