亲水作用色谱-串联质谱法测定婴儿配方乳粉中14 种母乳低聚糖

doi:10.7506/spkx1002-6630-20240409-072

食品科学 ›› 2025, Vol. 46 ›› Issue (3): 230-237.doi: 10.7506/spkx1002-6630-20240409-072

亲水作用色谱-串联质谱法测定婴儿配方乳粉中14 种母乳低聚糖

叶梦薇，黄芊，冯丽凤，黄媛，蔡小明，高宇，王天西，潘城

（国家加工食品质量检验检测中心，福建省产品质量检验研究院，福建福州 350002）

出版日期:2025-02-15 发布日期:2024-12-30
基金资助:
福建省市场监督管理局科技项目（FJMS2023015）；福建省产品质量检验研究院科技项目（KY202202A）

Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry

YE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng

(National Quality Supervision and Testing Center for Processed Food, Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China)

Online:2025-02-15 Published:2024-12-30

摘要/Abstract

摘要： 本研究建立了亲水作用色谱-串联质谱同时测定婴儿配方乳粉中14 种母乳低聚糖的分析方法。样品经乙醇沉淀蛋白、冷冻脱脂，以亲水作用色谱柱分离，采用超高效液相色谱-质谱多反应监测模式进行检测，外标法定量。在优化的条件下，14 种母乳低聚糖在各自的线性范围内线性关系良好，决定系数均大于0.994，在两种乳基试样的3 个不同加标水平下平均回收率为90.3%～109.2%、相对标准偏差为0.7%～9.7%（n＝6），方法检出限为0.002～0.048 μg/mL。此方法具有分析速度快、稳定性好、灵敏度高的特点，能够满足婴儿配方乳粉中14 种母乳低聚糖的快速检测需求。

关键词: 婴儿配方乳粉；母乳低聚糖；亲水作用色谱-串联质谱法

Abstract: A method for the determination of 14 human milk oligosaccharides (HMOs) in infant formula milk powder was established by hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The sample was deproteinized by ethanol precipitation and degreased by freezing prior to separation by HILIC. The analytes were detected by multiple reaction monitoring (MRM) and quantified by an external standard method. Under optimized conditions, all HMOs showed good linear relationships within their respective concentration ranges, with determination coefficients (R2) greater than 0.994. The average recovery rates from blank goat’s and cow’s milk powders at three spiked levels ranged from 90.3% to 109.2%, with relative standard deviation (RSD) in the range of 0.7%–9.7% (n = 6), and the limit of detection (LOD) between 0.002 and 0.048 μg/mL. The developed method is characterized by fast analysis speed, high sensitivity and good accuracy, and meets the requirements for the rapid detection of the 14 HMOs in infant formula milk powder.

Key words: infant formula milk powder; human milk oligosaccharides; hydrophilic interaction liquid chromatography-tandem mass spectrometry

中图分类号:

O629.1

叶梦薇，黄芊，冯丽凤，黄媛，蔡小明，高宇，王天西，潘城. 亲水作用色谱-串联质谱法测定婴儿配方乳粉中14 种母乳低聚糖[J]. 食品科学, 2025, 46(3): 230-237.

YE Mengwei, HUANG Qian, FENG Lifeng, HUANG Yuan, CAI Xiaoming, GAO Yu, WANG Tianxi, PAN Cheng. Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2025, 46(3): 230-237.

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亲水作用色谱-串联质谱法测定婴儿配方乳粉中14 种母乳低聚糖

Determination of 14 Human Milk Oligosaccharides in Infant Formula Milk Powder by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry

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