气相色谱-质谱结合香气活度值分析膜分离精制技术对桂花净油挥发性成分的影响

doi:10.7506/spkx1002-6630-20240529-255

食品科学 ›› 2025, Vol. 46 ›› Issue (5): 255-262.doi: 10.7506/spkx1002-6630-20240529-255

气相色谱-质谱结合香气活度值分析膜分离精制技术对桂花净油挥发性成分的影响

郭英杰，郭智鑫，卞建明，谢秋涛，李高阳，汪凯，郭佳婧，朱向荣

（1.湖南大学生物学院隆平分院，湖南长沙 410125；2.湖南省农业科学院农产品加工研究所，湖南长沙 410125；3.红牛维他命饮料（湖北）有限公司，湖北咸宁 437000）

出版日期:2025-03-15 发布日期:2025-02-07
基金资助:
湖南省农业科技创新资金项目（2023CX49）

Impact of Membrane Separation Refining on Volatile Organic Compounds of Osmanthus fragrans Absolute Oil Analyzed Using Gas Chromatography-Mass Spectrometry Combined with Odor Activity Value

GUO Yingjie, GUO Zhixin, BIAN Jianming, XIE Qiutao, LI Gaoyang, WANG Kai, GUO Jiajing, ZHU Xiangrong

(1. Longping Branch, College of Biology, Hunan University, Changsha 410125, China; 2. Agricultural Products Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 3. RedBull Vitamin Drink Co. Ltd., Xianning 437000, China)

Online:2025-03-15 Published:2025-02-07

摘要/Abstract

摘要： 为探究通过膜分离技术得到的精制桂花净油原液、截留液和透过液的香气品质差异，利用气相色谱-质谱技术对桂花净油进行检测，结果表明桂花净油样品中含有66 种挥发性成分（volatile organic compound，VOC），不同组分的VOC种类和含量存在显著差异（P＜0.05）。进一步应用正交偏最小二乘判别分析成功区分3 种不同的桂花净油组分，预测准确率达到93.6%。根据变量投影重要性值＞1筛选出了10 种差异性化合物，通过聚类热图分析出桂花净油原液、截留液和透过液含量突出的VOC分别为9、3、1 种；经过抗絮凝实验与流变学分析可知，桂花净油透过液具有良好的抗絮凝性与稳定的流变趋势，且其含有的棕榈酸及其衍生物含量最低。根据香气活度值（odor activity value，OAV）对各样品的香气成分进行评估，发现桂花净油原液样品的花香味、果香味、油脂香味和古龙样香气强度最为显著，其OAV分别为1 371 871.65、41 885.15、37 199.21和3 050.71；而桂花净油透过液样品的草香味强度最为突出，其OAV为3 241 379.60。本研究结果可为膜分离技术应用于纯化桂花净油以及桂花净油膜分离后各样品的特征香气评价提供理论依据。

关键词: 桂花净油；膜分离；挥发性成分；气相色谱-质谱；香气活度值

Abstract: In order to investigate the difference in aroma quality among Osmanthus fragrans absolute oil and the permeate and retenate obtained from its membrane separation, a total of 66 volatile organic compounds (VOCs) were detected in the samples by gas chromatography-mass spectrometry (GC-MS). Statistical analysis revealed significant difference in both the types and concentrations of VOCs among different samples (P < 0.05). The application of orthogonal partial least squares-discriminant analysis (OPLS-DA) successfully discriminated the three samples, with a predictive accuracy of 93.6%. In addition, 10 differential compounds were selected using variable important in the projection (VIP) value > 1 as the cutoff, and clustering heatmap analysis identified 9, 3 and 1 major VOCs in the oil, retenate and permeate, respectively. The permeate exhibited good anti-flocculation properties and a stable rheological tend, containing the lowest contents of palmitic acid and its derivatives. The absolute oil exhibited the most prominent floral, fruity, greasy and cologne-like aromas with odor activity value (OAV) of 1 371 871.65, 41 885.15, 37 199.21 and 3 050.71, respectively, whereas the permeate showed the most intense herbaceous aroma with OAV of 3 241 379.60. The results from the present study will provide a theoretical basis for the application of membrane separation to the purification of O. fragrans absolute oil and for the evaluation of the characteristic aroma of the oil and its fractions.

Key words: Osmanthus fragrans absolute oil; membrane separation; volatile organic compounds; gas chromatography-mass spectrometry; odor activity value

中图分类号:

TS221

郭英杰，郭智鑫，卞建明，谢秋涛，李高阳，汪凯，郭佳婧，朱向荣. 气相色谱-质谱结合香气活度值分析膜分离精制技术对桂花净油挥发性成分的影响[J]. 食品科学, 2025, 46(5): 255-262.

GUO Yingjie, GUO Zhixin, BIAN Jianming, XIE Qiutao, LI Gaoyang, WANG Kai, GUO Jiajing, ZHU Xiangrong. Impact of Membrane Separation Refining on Volatile Organic Compounds of Osmanthus fragrans Absolute Oil Analyzed Using Gas Chromatography-Mass Spectrometry Combined with Odor Activity Value[J]. FOOD SCIENCE, 2025, 46(5): 255-262.

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气相色谱-质谱结合香气活度值分析膜分离精制技术对桂花净油挥发性成分的影响

Impact of Membrane Separation Refining on Volatile Organic Compounds of Osmanthus fragrans Absolute Oil Analyzed Using Gas Chromatography-Mass Spectrometry Combined with Odor Activity Value

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