食品科学 ›› 2025, Vol. 46 ›› Issue (5): 281-291.doi: 10.7506/spkx1002-6630-20240731-302

• 包装贮运 • 上一篇    下一篇

贮藏条件对溏心蛋清凝胶特性影响及机制

向聪,钟梦真,霍嘉颖,李述刚,马璐璐   

  1. (1.广州市技师学院智慧农业产业系,广东 广州 510410;2.合肥工业大学食品与生物工程学院,安徽 合肥 230601)
  • 出版日期:2025-03-15 发布日期:2025-02-07
  • 基金资助:
    国家自然科学基金面上项目(32172226);“十四五”国家蛋鸡体系项目(JZ2024GQBK0161); 泓星优鹑食品海安有限公司合作项目(W2022JSKF0564);安徽荣达食品有限公司合作项目(W2020JSKF0489); 湖北宇祥畜禽有限公司合作项目(W2022JSKF0325);中央高校基本科研业务费专项(JZ2023HGQA0112)

Effects of Storage Conditions on Gel Characteristics of Soft-Boiled Egg White and Underlying Mechanisms

XIANG Cong, ZHONG Mengzhen, HUO Jiaying, LI Shugang, MA Lulu   

  1. (1. Department of Smart Agriculture Industry, Guangzhou Technician College, Guangzhou 510410, China; 2. School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China)
  • Online:2025-03-15 Published:2025-02-07

摘要: 本研究将新鲜鸡蛋在4 ℃和25 ℃条件下贮藏0~12 d后加热制成溏心蛋,研究贮藏条件对蛋清凝胶特性的影响,并探索其内在机制。结果表明,随着贮藏时间的延长,蛋清凝胶的质构特性、β-折叠的含量和二硫键均呈现先上升后下降的趋势,均在9 d时达到最高值,其中二硫键是分子间的主要作用力。与0 d比,在4 ℃条件下贮藏9 d时的蛋清凝胶回复性、弹性、β-折叠含量和二硫键变化显著,分别提升16.7%、29.9%、16.1%和22.1%;而25 ℃-9 d条件下分别提高34.7%、32.4%、30.4%和25.7%,且微观结构的孔洞更均匀、细密。因此,25 ℃-9 d组具有最好的凝胶特性。蛋白质组学表明25 ℃-9 d组中黏蛋白5B丰度为4 ℃-9 d组的6.4 倍、0 d组的11.4 倍,可能是其具有更好热凝胶特性的主要原因。

关键词: 贮藏条件;溏心蛋;蛋清热凝胶;蛋白质组学;黏蛋白5B

Abstract: In this study, the effects and underlying mechanisms of storage conditions on the gel properties of soft-boiled egg white prepared from fresh eggs stored at 4 or 25 ℃ for 0–12 days were investigated. The results showed that the texture properties, β-sheet content, and disulfide bond content of egg white gel initially increased and subsequently decreased with storage time, reaching the highest values after 9 days. Disulfide bonds served as the major intermolecular force. Compared with those of fresh samples, the resilience, springiness, β-sheet content, and disulfide bond content of soft-boiled egg white gels from eggs stored for 9 days increased by 16.7%, 29.9%, 16.1% and 22.1% for 4 ℃, and by 34.7%, 32.4%, 30.4% and 25.7% for 25 ℃, respectively, and the microstructure of the samples stored at 25 ℃ for 9 days was more uniform and compact, so it had the best gel properties. Proteomics analysis showed that the abundance of mucin 5B in the 25 ℃/9 days group was 6.4 and 11.4 times higher than that of the 4 ℃/9 days group and the fresh sample, respectively. which may account for its enhanced thermogel properties.

Key words: storage conditions; soft-boiled eggs; heat-induced egg white gels; proteomics; mucin 5B

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