基于广泛靶向代谢组学与高通量测序技术研究广陈皮原位仓储的陈化机制

doi:10.7506/spkx1002-6630-20241231-272

食品科学 ›› 2025, Vol. 46 ›› Issue (16): 33-43.doi: 10.7506/spkx1002-6630-20241231-272

基于广泛靶向代谢组学与高通量测序技术研究广陈皮原位仓储的陈化机制

何天行，苟海英，王彬，王福，陈鸿平，刘友平

（成都中医药大学药学院，西南特色中药资源国家重点实验室，四川?成都 611137）

出版日期:2025-08-25 发布日期:2025-07-22
基金资助:
国家自然科学青年科学基金项目（82104340）

Aging Mechanism of Pericarpium Citri Reticulatae ‘Chachiensis’ during in situ Storage Based on Widely Targeted Metabolomics and High-Throughput Sequencing

HE Tianxing, GOU Haiying, WANG Bin, WANG Fu, CHEN Hongping, LIU Youping

(State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China)

Online:2025-08-25 Published:2025-07-22

摘要/Abstract

摘要： 本研究探究原位仓储与非原位仓储广陈皮品质差异的形成机制。采用广泛靶向代谢组学及高通量测序技术解析原位仓储（广东）及非原位仓储（北京、四川、云南）陈化的广陈皮代谢物与微生物变化规律。超高效液相色谱-电喷雾离子化串联质谱共鉴定和表征了1 440 种代谢物，其中472 种化合物被筛选为差异代谢物；与其他陈化地点相比，于广东陈化的样品中黄酮类差异代谢物普遍上调，黄酮合成相关通路显著富集，并且总黄酮含量显著高于其他样品；14 种于广东陈化的样品中上调的黄酮类代谢物被确定为广陈皮陈化过程中的关键代谢物。微生物中Aspergillus、Sphingobium等物种在广东陈化样品中显著富集。相关性分析表明，气候因子中平均温度与关键差异代谢物呈显著正相关（P＜0.05），微生物Aspergillus、Cutibacterium、Staphylococcus、Xeromyces、Entyloma与6-甲氧基山柰酚-3-O-葡萄糖苷、2’,3’,4’,5,7-五羟基黄酮、黄芩黄酮II、槲皮素、金圣草黄素-7-O-龙胆二糖苷等关键代谢物呈显著正相关（P＜0.05），并筛选出Aspergillus、Xeromyces为陈化过程中的核心微生物。综上，平均温度作为广陈皮品质形成的重要影响因素，其诱导Aspergillus、Xeromyces等核心微生物富集，加速原位仓储的广陈皮中黄酮类化合物的积累。本研究可为阐明广陈皮原位仓储的意义以及深入理解广陈皮的陈化机制提供科学基础。

关键词: 代谢组学

Abstract: This study explored the mechanism of the differences in quality between in situ and non-in situ stored Pericarpium Citri Reticulatae ‘Chachiensis’, commonly known as Guangchengpi (GCP) in Chinese. Using widely targeted metabolomics and high-throughput sequencing, we analyzed the changes in metabolites and microbial communities in GCP during in situ (Guangdong) and non-in situ (Beijing, Sichuan, and Yunnan) storage. The results showed that ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) identified and characterized 1 440 metabolites, 472 of which were selected as differential metabolites. Compared with the other storage locations, all differential flavonoid metabolites identified in the samples stored in Guangdong were up-regulated, the flavonoid synthesis pathways were significantly enriched, and the total flavonoid content was significantly higher. Fourteen flavonoid metabolites upregulated in the Guangdong samples were identified as key metabolites in the aging process of GCP. Aspergillus and Sphingobium were significantly enriched in the Guangdong samples. Correlation analysis showed that the average temperature was significantly positively correlated with key differential metabolites (P < 0.05). Aspergillus, Cutibacterium, Staphylococcus, Xeromyces, Entyloma showed significantly positive correlations with the key metabolites 6-methoxykaempferol-3-O-glucoside, 2’,3’,4’,5,7-pentahydroxyflavone, skullcapflavone II, quercetin and chrysoeriol-7-O-gentiobioside (P < 0.05). Aspergillus and Xeromyces were selected as the core microorganisms during the aging process. In conclusion, the average temperature, as a significant factor affecting the quality of GCP, induces the enrichment of core microorganisms such as Aspergillus and Xeromyces, thus accelerating the accumulation of flavonoid compounds in GCP stored in situ. This study provides a scientific basis for elucidating the significance of in situ storage of GCP and gaining insights into its aging mechanism.

Key words: Pericarpium Citri Reticulatae ‘Chachiensis’

中图分类号:

TS201.3

何天行, 苟海英, 王彬, 王福, 陈鸿平, 刘友平. 基于广泛靶向代谢组学与高通量测序技术研究广陈皮原位仓储的陈化机制[J]. 食品科学, 2025, 46(16): 33-43.

HE Tianxing, GOU Haiying, WANG Bin, WANG Fu, CHEN Hongping, LIU Youping. Aging Mechanism of Pericarpium Citri Reticulatae ‘Chachiensis’ during in situ Storage Based on Widely Targeted Metabolomics and High-Throughput Sequencing[J]. FOOD SCIENCE, 2025, 46(16): 33-43.

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基于广泛靶向代谢组学与高通量测序技术研究广陈皮原位仓储的陈化机制

Aging Mechanism of Pericarpium Citri Reticulatae ‘Chachiensis’ during in situ Storage Based on Widely Targeted Metabolomics and High-Throughput Sequencing

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