食品科学 ›› 2025, Vol. 46 ›› Issue (16): 222-219.doi: 10.7506/spkx1002-6630-20250201-001

• 成分分析 • 上一篇    

人工接种发酵和自然发酵酸茶的风味差异分析

李琴芳,徐莹,汪东风,汪明明,白秀芳,李戈,李聪元   

  1. (1.中国海洋大学食品科学与工程学院,山东?青岛 266404;2.绿春县森泉茶叶厂,云南?红河哈尼族彝族自治州 662508;3.红河州经济作物技术推广站,云南?蒙自 661100)
  • 发布日期:2025-07-22
  • 基金资助:
    国家自然科学基金面上项目(32372287)

Analysis of Differences in Flavor between Sour Tea Produced by Inoculated and Natural Fermentations

LI Qinfang, XU Ying, WANG Dongfeng, WANG Mingming, BAI Xiufang, LI Ge, LI Congyuan   

  1. (1. College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China;2. Senquan Tea Factory, Lüchun County, Honghe Hani and Yi Autonomous Prefecture 662508, China; 3. Honghe Economic Crop Technology Promotion Station, Mengzi 661100, China)
  • Published:2025-07-22

摘要: 本研究探究外加菌株(肠膜明串珠菌和二孢接合酵母菌)对酸茶风味的影响,利用电子鼻、电子舌、气相色谱-离子迁移谱以及液相色谱-串联质谱技术结合感官评价分析酸茶的风味。结果表明,接种发酵酸茶的醇类、芳香类和有机硫化物类化合物均比自然发酵酸茶丰富;共检测出64 种挥发性风味化合物,筛选出17 种特征性化合物,其中在接种发酵酸茶中有9 种差异性风味化合物的相对含量高于自然发酵组,果香、酒香和木香突出。接种发酵酸茶的鲜味丰富性优于自然发酵酸茶,但涩味和酸味比自然发酵酸茶弱;检测出164 个差异代谢物,对滋味的形成有一定的影响。与自然发酵酸茶相比,接种发酵酸茶中有72 个代谢物相对含量显著增加,92 个代谢物相对含量显著减少。感官评价结果表明接种发酵酸茶风味更好、接受度更高。人工接种发酵显著提升了酸茶的风味品质,可为酸茶的开发生产提供新思路。

关键词: 酸茶;接种发酵;自然发酵;风味

Abstract: This study explored the effect of inoculated fermentation with Leuconostoc mesenteroides and Zygosaccharomyces bisporus on the flavor of sour tea. The flavor of sour tea was analyzed using an electronic nose, an electronic tongue, gas chromatography-ion mobility spectrometry (GC-IMS), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and sensory evaluation. Inoculated sour tea was richer in alcohols, aromatics, and organic sulfides than naturally fermented sour tea. A total of 64 volatile flavor compounds were detected, and 17 characteristic aroma components were identified. Among them, the relative contents of nine aroma components were significantly higher in inoculated tea than in naturally fermented tea, with the former exhibiting prominent fruity, winey, and woody aromas. Inoculated fermentation resulted in higher umami richness but astringency and sourness of sour tea compared with natural fermentation. A total of 164 differential metabolites were detected, which contributed to the taste formation of sour tea. Compared with naturally fermented sour tea, the relative contents of 72 metabolites were significantly increased, while the relative content of 92 metabolites were significantly decreased in inoculated sour tea. The sensory evaluation results indicated that the flavor of inoculated sour tea was superior and its acceptability was higher. This study showed that inoculated fermentation significantly improved the flavor quality of sour tea, providing a new approach for the development and production of sour tea.

Key words: sour tea; inoculated fermentation; natural fermentation; flavor

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