食品科学 ›› 2025, Vol. 46 ›› Issue (23): 160-168.doi: 10.7506/spkx1002-6630-20250609-047

• 生物工程 • 上一篇    

贺兰山东麓产区一株新型苹果酸-乳酸发酵细菌的酿酒适应性分析

祁璇,刘思,郑浩博,许引虎,马雯,金刚   

  1. (1.宁夏大学葡萄酒与园艺学院,宁夏 银川 750021;2.葡萄与葡萄酒教育部工程研究中心,宁夏 银川 750021;3.宁夏莱恩堡葡萄酒业有限公司,宁夏 银川 750200;4.宁夏大学化学化工学院,宁夏 银川 750021;5.农业微生物资源发掘与利用全国重点实验室,湖北 宜昌 443003;6.安琪酵母股份有限公司,湖北 宜昌 443003)
  • 发布日期:2025-12-26
  • 基金资助:
    宁夏回族自治区重点研发计划项目(2023BCF01027);宁夏自然科学基金项目(2023AAC03107)

Winemaking Adaptability of a Novel Malolactic Bacterial Strain from the Wine-Producing Region of the Eastern Foothill of Helan Mountain

QI Xuan, LIU Si, ZHENG Haobo, XU Yinhu, MA Wen, JIN Gang   

  1. (1. School of Enology and Horticulture, Ningxia University, Yinchuan 750021, China; 2. Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan 750021, China; 3. Ningxia Laienpu Wine Industry Co. Ltd., Yinchuan 750200, China; 4. School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China; 5. National Key Laboratory of Agricultural Microbiology, Yichang 443003, China; 6. Angel Yeast Co. Ltd., Yichang 443003, China)
  • Published:2025-12-26

摘要: 从宁夏贺兰山东麓产区青铜峡子产区赤霞珠干红葡萄酒自然发酵醪中分离纯化出一株乳酸菌,经16S rRNA序列分析及全基因组测序鉴定为希氏乳杆菌(Lentilactobacillus hilgardii),命名为Q19。乙醇耐受性分析结果显示:该菌能在含19%(V/V)乙醇的MRS培养基中存活且最高生长量可达OD600≈0.18,显示了其具有比目前生产中使用的不高于16%(V/V)乙醇耐受能力的商业菌株高得多的乙醇耐受能力。同时,以商业菌株酒酒球菌31-DH(Oenococcus oeni 31-DH)为对照,对L. hilgardii Q19的发酵速率,发酵前后葡萄酒基本理化指标、主要香气组分进行分析,结果表明:L. hilgardii Q19的发酵性能良好,发酵后葡萄酒部分指标优于O. oeni 31-DH,尤其在香气组成上,发酵后葡萄酒香气成分比O. oeni 31-DH多23 种,显著提升了葡萄酒风味复杂性。因此,L. hilgardii Q19具有很好的商业化应用潜力,特别是解决高糖高乙醇体积分数葡萄酒苹果酸-乳酸发酵面临的问题。

关键词: 希氏乳杆菌Q19;乙醇耐受性;酿酒学特性;香气组成

Abstract: In this study, a lactic acid bacteria was isolated and purified from naturally fermented Cabernet Sauvignon mash from the wine-producing region of Qingtong Gorge in the eastern foothill of Helan Mountain in Ningxia. It was identified as Lentilactobacillus hilgardii through 16S rRNA sequence analysis and whole genome sequencing and was named Q19. With respect to ethanol tolerance, L. hilgardii Q19 could survive in MRS medium with 19% (V/V) ethanol and grow up to an OD600 of 0.18, with its ethanol tolerance being higher than that of commercial wine strains, which can tolerate up to 16% (V/V) ethanol. Meanwhile, we compared L. hilgardii Q19 with the commercial Oenococcus oeni strain 31-DH in terms of fermentation rate, basic physicochemical indices, and major volatile compounds. The results showed that the fermentation performance of L. hilgardii Q19 was excellent. Some physicochemical indicators of wine fermented by this strain were better than those of wine fermented by O. oeni 31-DH, with the former containing 23 more volatile compounds and having a more complex aroma. Therefore, L. hilgardii Q19 has great potential for commercial use, particularly for addressing the challenges of malolactic fermentation (MLF) in high-ethanol wine making.

Key words: Lentilactobacillus hilgardii Q19; ethanol tolerance; oenological properties; aroma composition

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