食品科学 ›› 2026, Vol. 47 ›› Issue (1): 177-184.doi: 10.7506/spkx1002-6630-20250725-209

• 成分分析 • 上一篇    

基于中红外光谱技术的安吉金花茶发酵过程中关键成分含量快速检测

赵世茹,黄浦杰,李宪秀,毛旸晨,王珍珍,毛建卫,沙如意,邵逸怀   

  1. (1.浙江科技大学生物与化学工程学院,浙江 杭州 310023;2.安吉茶屿生物技术有限责任公司,浙江 湖州 313300)
  • 发布日期:2026-02-04
  • 基金资助:
    浙江省重点研发计划项目(2017C02009);省属高校基本科研业务费专项(2023JLYB007)

Mid-infrared Spectroscopy for Rapid Determination of Key Component Contents in Anji Golden Flower Tea during Eurotium cristatum Fermentation

ZHAO Shiru, HUANG Pujie, LI Xianxiu, MAO Yangchen, WANG Zhenzhen, MAO Jianwei, SHA Ruyi, SHAO Yihuai   

  1. (1. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China; 2. Anji Chayu Biotechnology Co. Ltd., Huzhou 313300, China)
  • Published:2026-02-04

摘要: 为提高安吉白茶修剪叶的利用率,实现对发酵茶主要品质成分的快速检测与定量分析,本研究通过冠突散囊菌(Eurotium cristatum)发酵安吉白茶修剪叶制备安吉金花茶,测定发酵过程中茶多酚、茶色素的含量变化,并基于傅里叶变换中红外光谱技术依次进行建模方法筛选、预处理方法筛选和光谱波段特征筛选,建立安吉金花茶发酵过程中茶多酚、茶色素含量的定量分析模型。结果表明,4 种成分含量均采用随机森林回归建立分析模型;茶多酚通过卷曲平滑预处理、迭代保留信息变量法特征筛选后,所构建的模型效果最优;茶黄素和茶红素模型均通过小波变换预处理,逐步投影算法筛选特征波段后,效果最优;茶褐素通过一阶导数+多元散射校正预处理、竞争性自适应重加权采样特征筛选后,所构建的模型效果最优。模型预测能力验证结果显示,4 种成分含量的预测值与实际值的平均偏差均低于0.05%,表明构建的定量分析模型效果较好,可实现对安吉金花茶中茶多酚、茶色素含量的快速检测。本研究基于傅里叶变换中红外光谱技构建出高效稳定的安吉金花茶中茶多酚与茶色素定量分析模型,为安吉白茶修剪叶高值化利用及发酵茶品质的快速检测提供了技术支撑。

关键词: 安吉白茶修剪叶;冠突散囊菌;发酵;中红外光谱;定量预测模型

Abstract: To enhance the utilization of pruned leaves from Anji white tea and to achieve rapid detection and quantitative analysis of key quality components in fermented tea, Eurotium cristatum was inoculated to pruned leaves of Anji white tea to prepare Anji golden flower tea, and the changes in the contents of tea polyphenols and tea pigments during the fermentation process were determined. Fourier transform mid-infrared spectroscopy was used to develop quantitative analysis models for the contents of tea polyphenols and tea pigments during the fermentation process. For this purpose, optimization of modeling algorithms, optimization of spectral preprocessing techniques, and feature wavelength selection were carried out. Results showed that random forest regression (RFR) was selected to establish predictive models for the contents of tea polyphenols and tea pigments. The predication model of tea polyphenols obtained using Savitzky-Golay smoothing (SG) combined with iteratively retains informative variables (IRIV) showed the best performance. The optimal spectral preprocessing and variable selection methods were wavelet transform (WT) and successive projections algorithm (SPA) for both theaflavins and thearubigins, while those for theabrownins were first derivative (FD) + multiplicative scatter correction (MSC) and competitive adaptive reweighted sampling, respectively. The models’ predictive performance was validated, revealing that the average deviations between predicted and actual values were less than 0.05% for all four tea quality components. Therefore, the quantitative analysis models exhibited good accuracy, enabling rapid detection of the contents of tea polyphenols and tea pigments in Anji golden flower tea. They provide technical support for the high-value utilization of pruned leaves from Anji white tea and the rapid quality monitoring of fermented tea products.

Key words: pruned leaves of Anji white tea; Eurotium cristatum; fermentation; mid-infrared spectroscopy; quantitative analysis models

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