食品科学 ›› 2010, Vol. 31 ›› Issue (21): 204-207.doi: 10.7506/spkx1002-6630-201021046

• 生物工程 • 上一篇    下一篇

温度和乳基质环境对EPEC O114:K90存活的影响

姜 博,孟祥晨* ,张红宇   

  1. 乳品科学教育部重点实验室,东北农业大学食品学院
  • 收稿日期:2010-01-19 修回日期:2010-08-24 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 孟祥晨 E-mail:xchmeng@163.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD04A08)

Effects of Storage Temperature and Milk-based Media on Survival of Enteropathogenic Escherichia coli EPEC O114:K90

JIANG Bo,MENG Xiang-chen*,ZHANG Hong-yu   

  1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University,
    Harbin 150030, Ch
  • Received:2010-01-19 Revised:2010-08-24 Online:2010-11-15 Published:2010-12-29
  • Contact: MENG Xiang-chen E-mail:xchmeng@163.com

摘要:

探讨肠致病性大肠杆菌EPEC O114:K90 在不同温度和乳基质环境中的存活情况。以一株分离自原料乳的肠致病性大肠杆菌EPEC O114:K90 为研究对象,采用人工污染方法,将该菌以不同初始浓度接种到乳基质(灭菌乳、酸乳及乳酸菌饮料)中,通过平板菌落计数方法确定温度(4、10、25、72℃水浴15min)和乳基质环境对该菌存活的影响。结果表明:低温和低酸性环境能够减缓EPEC O114:K90 的生长,72℃水浴15min 巴氏杀菌能够完全使乳基质中的EPEC O114:K90 失活,该菌对正常的加工条件比较敏感。

关键词: EPEC O114:K90, 温度, 存活, 酸乳

Abstract:

In this study, the survival situation of enteropathogenic Escherichia coli EPEC O114:K90 in different milk-based products stored at different temperatures was explored. Sterile milk, yoghurt and sour milk bacteria beverage were artificially contaminated by different initial concentrations of EPEC O114:K90 and subsequently stored at different temperatures. The survival rate of EPEC O114:K90 was periodically measured by plate count method during storage. Both low temperature and low acid environment could alleviate the growth of EPEC O114:K90. Water bath treatment at 72 ℃ for 15 min completely inactivated EPEC O114:K90 in the above three milk-based products. This strain was sensitive to regular processing conditions.

Key words: EPEC O114:K90, temperature, survival, yogurt

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