外界因素对重组enterocin A抗菌活性的影响

doi:10.7506/spkx1002-6630-201017073

食品科学 ›› 2010, Vol. 31 ›› Issue (17): 327-330.doi: 10.7506/spkx1002-6630-201017073

外界因素对重组enterocin A抗菌活性的影响

赵爱珍¹,徐兴然¹,韩文瑜²

1. 西南大学药学院
2. 吉林大学畜牧兽医学院

收稿日期:2010-06-07 出版日期:2010-09-15 发布日期:2010-12-29
通讯作者: 赵爱珍 E-mail:zaoaizhen@yahoo.com.cn

Effects of External Factors on Anti-Listeria LIN 3 Activity of Recombinant Enterocin A

ZHAO Ai-zhen1，XU Xing-ran1，HAN Wen-yu2

1. College of Pharmaceutical Science, Southwest University, Chongqing 400715, China；
2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China

Received:2010-06-07 Online:2010-09-15 Published:2010-12-29
Contact: ZHAO Ai-zhen E-mail:zaoaizhen@yahoo.com.cn

摘要/Abstract

摘要：

探讨蛋白酶、还原剂、温度、pH 值4 个外界因素对Enterocin A 抗菌活性的影响。对重组Enterocin A 进行不同处理后，采用琼脂孔扩散实验、琼脂点种实验或者平板菌落计数法检测样品的抗李斯特菌活性。结果表明：重组Enterocin A 的蛋白酶消化产物的抗菌活性丧失，其β - 巯基乙醇还原产物的抗菌活性也丧失；重组EnterocinA 表现较高的热稳定性，100℃处理10min 后仍可以检测到抗菌活性；该重组细菌素在pH2.0～8.0 条件下均保持抗菌活性。

关键词: 重组enterocin A, 抗李斯特菌活性, 外界因素

Abstract:

With and without sole exposures to proteases (trypsin and protease K), reductant (2-mercaptoethanol), temperature and pH, recombinant Enterocin A was tested for its anti-Listeria LIN 3 activity by agar diffusion, agar spot or plate count methods in order to figure out the effects of these external factors on this biological activity. Due to protease hydrolysis, recombinant Enterocin A thoroughly lost its anti-Listeria LIN 3 activity. The presence of β-mercaptoethanol also resulted in a near-complete loss of its anti-Listeria LIN 3 activity. Recombinant Enterocin A presented high thermal stability. In spite of heating for 10 min at 100 ℃, its anti-Listeria LIN 3 activity could still be detected. Recombinant Enterocin A could maintain anti-Listeria LIN 3 activity in the pH range from 2.0 to 8.0.

Key words: recombinant Enterocin A, anti-Listeria LIN 3 activity, external factors

中图分类号:

TS201.3

赵爱珍，徐兴然，韩文瑜. 外界因素对重组enterocin A抗菌活性的影响[J]. 食品科学, 2010, 31(17): 327-330.

ZHAO Ai-zhen1，XU Xing-ran1，HAN Wen-yu2. Effects of External Factors on Anti-Listeria LIN 3 Activity of Recombinant Enterocin A[J]. FOOD SCIENCE, 2010, 31(17): 327-330.

[1]	赵爱珍，徐兴然. Enterocin A构效关系的研究[J]. 食品科学, 2012, 33(19): 269-272.
[2]	赵爱珍，徐兴然，韩文瑜. 二硫键对Enterocin A抗李斯特菌LIN 3活性的影响[J]. 食品科学, 2011, 32(15): 182-185.

外界因素对重组enterocin A抗菌活性的影响

Effects of External Factors on Anti-Listeria LIN 3 Activity of Recombinant Enterocin A

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价