大孔吸附树脂分离纯化峨嵋岩白菜叶总黄酮

doi:10.7506/spkx1002-6630-201006017

食品科学 ›› 2010, Vol. 31 ›› Issue (6): 74-79.doi: 10.7506/spkx1002-6630-201006017

大孔吸附树脂分离纯化峨嵋岩白菜叶总黄酮

陈蕉¹,黎云祥¹,陈光登¹,蔡凌云^1.2

1.西华师范大学生命科学学院，四川省高校环境科学与生物多样性保护重点实验室 2.凯里学院环境与生命科学学院

收稿日期:2009-03-25 修回日期:2009-10-18 出版日期:2010-03-15 发布日期:2010-12-29
通讯作者: 黎云祥 E-mail:yx_li@263.net
基金资助:
四川省科技厅应用基础研究项目(2008JY0111)；四川省杰出青年学科带头人培养计划项目(04ZQ026-047)；
四川省重点学科建设项目(SZD0420)

Macroporous Resin Adsorption for Purification of Total Flavonoids from Bergenia emeiensis CY Wu Leaves

CHEN Jiao1，LI Yun-xiang1,*，CHEN Guang-deng1，CAI Ling-yun1,2

1. College of Life Science, China West Normal University, Key Laboratory of Environmental Science and Biodiversity
Conservation of Sichuan Higher Education, Nanchong 637002, China；2. School of Envirimental and Life Science, Kaili
University, Kaili 556003, China

Received:2009-03-25 Revised:2009-10-18 Online:2010-03-15 Published:2010-12-29
Contact: LI Yun-xiang1 E-mail:yx_li@263.net

摘要/Abstract

摘要：

目的：筛选出分离纯化峨嵋岩白菜叶总黄酮的最佳大孔树脂型号及工艺条件。方法：通过静态、动态相结合的方法，以黄酮吸附率、解吸率为指标，确定最佳工艺。结果：HPD-600 型树脂具有最佳的吸附和洗脱参数，其最佳工艺为：粗提物水溶液上样总黄酮质量浓度为0.5～0.6mg/mL，上样液pH3～4，吸附流速2mL/min，最大上样量为3BV。吸附后先以3BV 水洗去杂质，再用4BV 的90% 乙醇以2mL/min 的速率进行洗脱，黄酮纯度和回收率分别达29% 和80% 以上。结论HPD-600 可较好地吸附分离峨嵋岩白菜叶总黄酮，纯化后黄酮纯度提高5倍以上，且操作简单、安全、成本低廉，有较高的应用价值。

关键词: 大孔吸附树脂, 峨嵋岩白菜叶, 总黄酮, 分离纯化

Abstract:

An optimal macroporous resin and its optimal technological parameters for purification of crude total flavonoids separated from Bergenia emeiensis CY Wu leaves were investigated in the present study. HPD-600 resin was chosen due to its excellent adsorption and desorption performance. Static and dynamic adsorption and desorption behaviors of HPD-600 resin were evaluated for achieving an optimal purification of total flavonoids. Results showed that the optimal purification process was that sample solution at pH 3－4 and the concentration level of 0.5－0.6 mg/mL was made to flow through HPD-600 resin column with a maximum injection volume of 3 BV and subsequently, the column was washed with 3 BV of water for the removal of impurities and eluted with 4 BV of 90% ethanol at a flow rate of 2 mL/min. More than 80% total flavonoids with a purity of more than 29% were recovered using the above process. These data demonstrate the excellence of HPD-600 resin in purifying total flavonoids from Bergenia emeiensis CY Wu leaves and the advantages of the developed method for industrial application, like simple operation, safety, and low cost.

Key words: macroporous adsorption resin, B. emeiensis leaves, total flavonoids, purification

中图分类号:

R284.2

陈蕉1，黎云祥1,*，陈光登1，蔡凌云1,2. 大孔吸附树脂分离纯化峨嵋岩白菜叶总黄酮[J]. 食品科学, 2010, 31(6): 74-79.

CHEN Jiao1，LI Yun-xiang1,*，CHEN Guang-deng1，CAI Ling-yun1,2. Macroporous Resin Adsorption for Purification of Total Flavonoids from Bergenia emeiensis CY Wu Leaves[J]. FOOD SCIENCE, 2010, 31(6): 74-79.

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大孔吸附树脂分离纯化峨嵋岩白菜叶总黄酮

Macroporous Resin Adsorption for Purification of Total Flavonoids from Bergenia emeiensis CY Wu Leaves

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