FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (20 ): 260-264.doi: 10.7506/spkx1002-6300-200920055

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Purification of Polyphenols from Gorgon Nut Using D101-type Macroporous Adsorption Resin

ZHANG Cuan,XUE Lian-hai,JIA Xiao-li,SUN Yan-hui   

  1. Department of Chemistry and Life Science, Chuzhou University, Chuzhou 239012, China
  • Received:2009-07-16 Online:2009-10-15 Published:2010-12-29
  • Contact: ZHANG Cuan E-mail:zhangchuan2005@126.com

Abstract:

Crude polyphenols was isolated from gorgon nut and purified using D101-type resin adsorption-column chromatography with elution of 50% acetone aqueous (V/V) at pH 5. Results revealed that D101-type resin exhibited a high adsorption capacity (33.21 mg/g) to gorgon nut polypenols (GNPP). High adsorption specificity to GNPP was observed in D101 resin compared with impurities in the mixture solution such as proteins and carbohydrates. Under optimal purification conditions, 91.84% GNPP could be eluted from D101-type resin and the purity of polyphenols was 81.38% through condensation at reduced pressure.

Key words: gorgon nut polyphenols (GNPP), adsorption characteristics, isolation, purification

CLC Number: