FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (20 ): 331-335.doi: 10.7506/spkx1002-6300-200920073

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Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of Nitrofuran Metabolites Residues in Aquatic Products

LIU Si-xin1,HU Xian-e1,LIN Zheng-feng2,HE Xue-lian2,LIU Hong-zhuan2,LI Cong-fa1,*   

  1. 1. College of Food Science and Technology, Hainan University, Haikou 570228, China;
    2. Technical Center, Hainan Exit-Entry Inspection and Quarantine Bureau, Haikou 570311, China
  • Received:2009-05-26 Online:2009-10-15 Published:2010-12-29
  • Contact: LI Cong-fa

Abstract:

The Chinese national standard method, GB/T 20752—2006 has been used for the determination of nitrofuran metabolites residues in aquatic products using liquid chromatography-tandem mass spectrometry (LC-MS/MS), with a recovery rate of 40% and a linear range of 0.2-2 μg/kg. Extraction of test portions with ethyl acetate followed by clean-up using liquid-liquid extraction was adopted and appropriate adjustments of LC-MS/MS conditions were conducted as improvements for the method. Results showed that the pretreatment time was shortened, and the average spike recovery rates for six replicate determinations were elevated to 81.3%-106.0%, with a RSD of less than 11%. This method exhibited a linear range of 0.1-4 μg/kg (r2 > 0.999) and a limit of detection of 0.1  μg/kg.

Key words: nitrofuran metabolites residues, liquid chromatography-tandem mass spectrometry (LC-MS /MS), aquatic products

CLC Number: