Abstract:

In order to effectively detect minocycline (MNC) residues, a complete antigen of MNC was developed by glutaraldehyde synthesis and diazotization methods. MNC was coupled with bovine serum albumin (BSA) to obtain a complete antigen of MNC-BSA. Infrared and ultraviolet scanning spectral analysis confirmed that the complete antigen of MNC-BSA had been successfully coupled by both methods, with average conjugation ratios of 9.9 and 5.9, respectively. The serum titer of rabbit immunized with MNC-BSA competent antigen prepared by glutaraldehyde synthesis method was 16000, which indicated that the synthesized complete antigen could not only be used as the immunogen to prepare the corresponding antibody, but also act as the coating antigen for enzyme linked immunosorbent assay (ELISA) kit.

Key words: minocycline, bovine serum albumin (BSA), conjugation, complete antigen