Abstract:

A preparation method of high purity Salidroside from Rhodida rosea was investigated in this paper. The crude extract of Rhodida rosea was subjected to 75% ethanoll distilment, acetic plumbum precipitation and n-butanol extraction and the content of salidroside was 43.80%. The product was further adsorbed by macroporous resin (D101 and AB-8) and silica gel and qualitative and quantitative analysis were performed by TLC and RRLC. It was showed that the product after column chromatogram of AB-8 and elution by water and 5% ethanol, salidroside with high purity over 90% was obtained. This method was proved to the most effecient for preparation of salidroside from Rhodida rosea in large-scale.

Key words: salidroside, separation and purification, column chromatogram