Rapid Detection of Live/Dead Salmonella via EMA-LAMP Method

doi:10.7506/spkx1002-6300-200922077

Abstract

Abstract:

A combinatorial method, designated as ethidium monoazide (EMA)-loop-mediated isothermal amplification (LAMP) method, was established to detect live/dead Salmonella due to the selective penetration of EMA into dead cells in the presence of live cells. DNA covalently bound to EMA can not be amplified by LAMP. In this detect system, a series of primers were specially designed to recognize six distinct sequences on target invA gene in Salmonella. Totally 241 bp target DNA was amplified and visualized as ladder-like bands on agarose gel within 60 min under isothermal condition of 65 ℃. This detection method was rapid, convenient and highly sensitive. The detection limit of this LAMP assay was 10-10 g DNA/tube, which exhibited 100- fold higher sensitivity than EMA-PCR method. This novel EMA-LAMP method for detecting live/dead bacteria will have great potential.

Key words: Salmonella, rapid detection, EMA-LAMP

CLC Number:

TS207.4

LU Yu-xia，GUO Si-yuan，SHI Lei*，LI Lin. Rapid Detection of Live/Dead Salmonella via EMA-LAMP Method[J]. FOOD SCIENCE, 2009, 30(22): 324-327 https://doi.org/10.7506/spkx1002-6300-200922077

[1]	HU Xinbo, YUAN Fei, LÜ Hongrui, CAI Jie, LI Tao, LI Hongna, ZHANG Yinghao, SUN Dongmei, YANG Yange. Recent Progress on Technologies for Diuretic Detection in Foods and Functional Foods [J]. FOOD SCIENCE, 2026, 47(3): 366-376.
[2]	GAN Hongxiang, XU Anning, ZHOU Lu, LUO Fangfang, FENG Zhongyang, GAO Binru, LI Zhuosi, WANG Xiang, DONG Qingli, QIN Xiaojie. Comparative Analysis of Genomic Characteristics and Virulence Factors of Salmonella Typhimurium Monophasic Variant Isolates from Foods and Humans [J]. FOOD SCIENCE, 2025, 46(6): 116-123.
[3]	LI Fangshu, ZHANG Ruiting, CHENG Zan, HU Guangyue, MA Yuanye, DAI Qin, KANG Rui, LI Xin, SHE Yongxin, WANG Shanshan. Recent Advances in Rapid Detection Technologies Based on Precise Recognition for Pesticide and Veterinary Drug Residues [J]. FOOD SCIENCE, 2025, 46(6): 330-343.
[4]	WANG Yuhang, ZHOU Lingling, ZHOU Yaoling, SA Junmeng, ZHANG Yuanchen, MA Zengjun, LIAN Kaiqi. Antimicrobial Mechanism of Antimicrobial Peptide LL-1 against Salmonella [J]. FOOD SCIENCE, 2025, 46(4): 110-116.
[5]	GAO Jianxin, WANG Xin, ZHANG Hongyan. A Dual-Mode Aptamer-Based Lateral Flow Test Strip for Rapid Detection of Salmonella typhimurium Based on the Photothermal and Catalytic Effects of Fe3O4@Au Composite Nanomaterials [J]. FOOD SCIENCE, 2025, 46(24): 44-52.
[6]	WEI Qing, LIU Yan, LIU Huilin. Functionalized Nanomaterials Promote Rapid Analysis of New Contaminants in Foods: from Design to Application [J]. FOOD SCIENCE, 2025, 46(24): 53-64.
[7]	ZHOU Haibo, LIU Xinmei, WANG Na, LIU Ning, YANG Jun. Simultaneous Detection of Vibrio parahaemolyticus and Tetracycline Resistance Genes Using Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Technology [J]. FOOD SCIENCE, 2025, 46(24): 313-320.
[8]	XU Zhenlin, LIANG Xiaomin, LIU Minling, AORI Qileng, SHEN Xing, WANG Hong. Nanobody-Based Strategies for Rapid and Intelligent Detection of Foodborne Contaminants: Progress and Perspectives [J]. FOOD SCIENCE, 2025, 46(23): 1-12.
[9]	XIE Xinna, QIU Manyan, ZHANG Xiru, ZHU Danqing, ZHANG Jingwen, YANG Xinyan, JIANG Yujun, MAN Chaoxin, ZHANG Xianlong. Development of a CRISPR/Cas12a-Based Fluorescence Detection Method for Pseudomonas fluorescens in Dairy Products [J]. FOOD SCIENCE, 2025, 46(23): 13-20.
[10]	LIU Nuoyu, GAO Xing, LIANG Haiyang, HU Jinwei, ZHAO Zhifeng, ZHOU Yu, XU Weizhen. Rapid Detection of Cyanide in Baijiu by Highly Sensitive Colorimetric Hydrogel Sensors with Au-AgI Dimer Nanoparticles [J]. FOOD SCIENCE, 2025, 46(22): 321-331.
[11]	YUAN Yuhang, LIAN Jie, SHAO Zhenchao, ZENG Dawei, WANG Huichao, LI Meng. Ambient Mass Spectrometry and Ambient ionization Mass Spectrometry Imaging in Rapid Food Safety Detection [J]. FOOD SCIENCE, 2025, 46(19): 290-298.
[12]	YUE Xiaoyue, YAN Shuliang, FU Long, ZHAO Dianbo. Colorimetric Method Based on Cerium Dioxide/Iron Oxyhydroxide Nanozyme for Rapid Detection of Hydrogen Peroxide in Chicken Feet with Pickled Pepper [J]. FOOD SCIENCE, 2025, 46(1): 166-175.
[13]	JIN Yan, WANG Jingyi, CHENG Jianing, YU Lemin, ZHANG Bichen, ZHANG Yibo, XU Tongyu. Immunomagnetic Separation of Salmonella Typhimurium Based on Microfluidic Chip [J]. FOOD SCIENCE, 2025, 46(1): 200-209.
[14]	LIN Zhiwei, WANG Shuai, CAI Jie, NIE Dandan, LI Tao, LI Hongna, YANG Yange, YUAN Fei. Establishment of Three Rapid Visual Detection Methods for Burkholderia gladioli pv. cocovenenans Based on Body Temperature Amplification [J]. FOOD SCIENCE, 2024, 45(9): 219-225.
[15]	YUE Xiaoyue, WU Chaoyun, YAN Shuliang, FU Long, ZHAO Dianbo. Colorimetric Method Based on CeO2/CoOOH Nanozyme for Rapid Detection of Ascorbic Acid in Fruits [J]. FOOD SCIENCE, 2024, 45(24): 250-258.

Rapid Detection of Live/Dead Salmonella via EMA-LAMP Method

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments