Abstract:

The volatile oil composition of seeds (SPF) and leaves (LPF) of Perilla frutescens (L.) Britt. was investigated by two-dimensional gas chromatography-mass spectrometry (GC-MS). The overlapped spectral data of SPF and LPF volatile oils were resolved by chemometric method into a pure chromatogram and a mass spectrum of each compound, which was qualified by mass spectral library search. In total, 85 volatile chemical components from SPF and 74 from LPF were identified, which accounted for 97.92% and 98.56% of total volatile oil, respectively. Forty-five volatile components were observed in both SPF and LPF, occupying 52.94% and 60.81% of total numbers of volatile oil components identified in SPF and LPF, occupying 84.30% and 66.38% of total contents, respectively. The dominant common volatile chemical components in SPF and LPF were D-Limonene, bicyclo[7.2.0]undec-4-ene,4,11,11-trimethyl-8-methylene-Bicyclo[3.1.1]hept-2-ene, 2,6-dimethyl-6-(4-methyl- 3-pentenyl)-, caryophyllene oxide, 1-pentanone, 1-(2-furanyl)- and tricyclo[3.1.0.0(2,4)]hexane,3,6-diethyl-3,6-dimethyl-.

Key words: seeds of Perilla frutescens (SPF), leaves of Perilla frutescens (LPF), volatile oil, gas chromatography-mass spectrometry (GC-MS), chemometric resolution method (CRM)