Isolation and Characterization of Polysaccharides from Momordica charantia L. Fruits

doi:10.7506/spkx1002-6300-201003007

Abstract

Abstract:

Crude polysaccharides (MCP) were extracted from dried fruits of Momordica charantia L. through sequential steps of hot water extraction, ethanol precipitation and Sevag protein removal and purified/fractionated by DEAE-cellulose-52 exchange chromatography to obtain four fractions (MCP-A, MCP-B, MCP-C and MCP-D). MCP-A1 with high purity was obtained through further purification of MCP-A by Superdex G-100 gel filtration chromatography and its purity was identified by high performance gel permeation chromatography (HPGPC). Results indicated MCP-A1 was a homogeneous polysaccharide with average molecular weight of 93577 D. Qualitative chemical reaction showed that MCP-A1 was a neutral polysaccharide that contained uronic acid, rather than proteins, nucleic acids and starch. Typical absorption peaks of this polysaccharide were also observed in infrared (IR) spectrum.

Key words: Momordica charantia L., polysaccharide, isolation, purification, characterization

CLC Number:

Q946.3

LIU Guo-ling1，NING Zheng-xiang2，GUO Hong-hui1. Isolation and Characterization of Polysaccharides from Momordica charantia L. Fruits[J]. FOOD SCIENCE, 2010, 31(3): 30-34 https://doi.org/10.7506/spkx1002-6300-201003007

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Isolation and Characterization of Polysaccharides from Momordica charantia L. Fruits

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