Orthogonal Array Design Tests for Optimizing Ultrasound-assisted Extraction of Black Rice Pigment

doi:10.7506/spkx1002-6300-201004009

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (4): 39-41.doi: 10.7506/spkx1002-6300-201004009

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Orthogonal Array Design Tests for Optimizing Ultrasound-assisted Extraction of Black Rice Pigment

ZHANG Ji-xiang1，ZHAO Wen-jing1，BAI Xia-jie1，OU Lai-liang2,*

1. Faculty of Chemistry and Material Science, Langfang Teachers College, Langfang 065000, China；2. Key Laboratory of
Bioactive Materials, Ministry of Education, Institute of Molecular Biology, Nankai University, Tianjin 300071, China

Received:2009-04-27 Online:2010-02-15 Published:2010-12-29
Contact: OU Lai-liang E-mail:zhangjixiang1973@yahoo.com.cn

Abstract

Abstract:

Ethanol was used for extracting black rice pigment under ultrasound assistance. To maximize the extraction of black rice pigment, orthogonal array design was employed for investigating the effects of ethanol concentration, solid-to-liquid ratio, ultrasound power, extraction time on black rice pigment yield (represented by the absorbance at 543 nm wavelength of extract solution). Results showed that the use of 80% ethanol for extracting black rice grains at 1:32 solid-to-liquid ratio (m/V) with the assistance of 50 min ultrasound treatment at 250 W gave an optimal pigment extraction and the resulting pigment yield reached up to 4.5%, with a near 3-fold increase in comparison with the conventional Soxhlet extraction.

Key words: black rice, pigment, orthogonal array design, ultrasound-assisted extraction

CLC Number:

TS202.3

ZHANG Ji-xiang1，ZHAO Wen-jing1，BAI Xia-jie1，OU Lai-liang2,*. Orthogonal Array Design Tests for Optimizing Ultrasound-assisted Extraction of Black Rice Pigment[J]. FOOD SCIENCE, 2010, 31(4): 39-41.

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Orthogonal Array Design Tests for Optimizing Ultrasound-assisted Extraction of Black Rice Pigment

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