Abstract:

To optimize the microwave-assisted extraction process of Pleurotus ostreatus polysaccharide (POP), single factor experiments involving three technological parameters including extraction time, microwave power and solvent/material ratio were done for following investigations on the interactive effects of the parameters on POP yield using central composite design (CCD) combined with response surface methodology. In the optimization, Design Expert 7.1.6 software was used to fit CCD experimental data and a quadratic polynomial regression model was developed for POP yield as a response to the above parameters. Results showed that the optimal values of technological parameters for using deionized water to extract POP were as follows: extraction time 10 min, microwave power 420 W and solvent/material ratio 40:1 (mL/g). Under such conditions, the yield of POP was up to 9.04%.

Key words: Pleurotus ostreatus polysaccharide, microwave-assisted extraction, response surface analysis