Purification of Saponin from Edible Stems of Aralia continentalis Using Macroporous Adsorption Resin

doi:10.7506/spkx1002-6300-201004016

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (4): 73-76.doi: 10.7506/spkx1002-6300-201004016

• Processing Technology • Previous Articles Next Articles

Purification of Saponin from Edible Stems of Aralia continentalis Using Macroporous Adsorption Resin

FENG Ying1,2，WU Mei-ling1，LI Tian-lai2,*，FAN Wen-li2

1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China；
2. College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China

Received:2009-03-19 Revised:2009-09-11 Online:2010-02-15 Published:2010-12-29
Contact: FENG Ying E-mail:fywjg@sina.com

Abstract

Abstract:

Static adsorption and desorption experiments were carried out to screen an optimal one from 4 types of macroporous adsorption resins for the purification of saponin from edible stems of Aralia continentalis. AB-8-type macroporous resin was shown to be an optimum candidate for saponin purification. Subsequently, dynamic adsorption and desorption experiments were performed to optimize technological conditions for saponin purification using AB-8-type macroporous resin. Results showed that the optimal adsorption conditions were obtained as follows: saponin concentration 0.2 mg/mL and sample injection amount 20-fold bed volume (BV). Two highly pure saponin fractions having strong superoxide anion radical scavenging capacity and antibacterial potential were obtained from gradient elution with 50% and 70% ethanol, respectively.

Key words: Aralia continentalis, saponin, macroporous adsorption resin

CLC Number:

Q946.83

FENG Ying1,2，WU Mei-ling1，LI Tian-lai2,*，FAN Wen-li2. Purification of Saponin from Edible Stems of Aralia continentalis Using Macroporous Adsorption Resin[J]. FOOD SCIENCE, 2010, 31(4): 73-76.

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Purification of Saponin from Edible Stems of Aralia continentalis Using Macroporous Adsorption Resin

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