Abstract:

A new analytical approach using HPLC-MS was developed for the determination of chloramphenicol residue in meat. Samples were extracted with ethyl acetate, and the resulting extract was blown to dryness under nitrogen. Subsequently, the residue left was dissolved in water, defatted with n-hexan. Finally, the water phase was harvested and filtrated through 0.2μm membrane for HPLC-MS analysis. The chromatographic separation was achieved on a C18 column with a mobile phase consisting of acetronitrile and 0.1% acetate. ESI(-)-SIM mode was adopted for the detection of chloramphenicol. The developed approach is characterized by short time consumption for chromatographic separation, high sensitivity and good reliability so as to meet the requirements for rapid analysis of large-batch samples.

Key words: chloramphenicol, HPLC-MS, analysis