Abstract:

Based on the investigations of artificial coating antigen for chlorpyrifos and polycolonal antibody against chlorpyrifos, an indirect ELISA kit was developed for detecting chlorpyrifos residue in plant-derived foods. In addition, the developed kit was evaluated for sensitivity, specificity, precision, accuracy and expiration date. Results indicated that the limit of detection of the kit was 0.5μg/L and the linear range was between 1.8μg/L and 1000 μg/L. Both intra-assay and inter-assay coefficients of variation were less than 8%. Mean spike recoveries of chlorpyrifos in tap water, lettuce, apple and rice were all more than 85%. The kit stored at 4 ℃ or －20 ℃ exhibited more than 6-month expiration date.

Key words: chlorpyrifos, residue, enzyme linked immuno-sorbent assay (ELISA), kit