FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 23-25.doi: 10.7506/spkx1002-6300-201005006

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Antioxidant Activity of Extract from Balanophora spicata Hayata in vitro

DENG Jing1,2,MO Zheng-chang1,2,JI Guang-quan1,2,YANG Juan2,*   

  1. 1. College of Life Science, Guizhou University, Guiyang 550025, China;2. The Key Laboratory of Chemistry for Natural
    Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China
  • Received:2009-06-09 Online:2010-03-01 Published:2010-12-29
  • Contact: YANG Juan2,* E-mail:yangxz2002@126.com;linyj401@tom.com

Abstract:

DPPH· free radical scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of ethanol extract, petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract, water extraction fraction, water-soluble crude polysaccharides and alkaline-soluble crude polysaccharides from Balanophora spicata Hayata. Results indicated that the extracts from Balanophora spicata Hayata all exhibited strong scavenging capacity against DPPH free radicals, especially ethyl acetate extract, n-butanol extract, ethanol extract and water-soluble crude polysaccharides, which were nearly equivalent to vitamin C for DPPH free radical scavenging activity. The fact that ethyl acetate extract exhibited an IC50 value of 6.0 μg/mL suggested that a little stronger antioxidant activity of ethyl acetate extract than vitamin C. Meanwhile, the above extracts all revealed stronger ferric reducing power.

Key words: Balanophora spicata Hayata, antioxidant activity, DPPH free radical scavenging activity, reducing power

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