FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 63-67.doi: 10.7506/spkx1002-6300-201005015
• Basic Research • Previous Articles Next Articles
LIN Lian-zhu,ZHAO Mou-ming*
Received:
Revised:
Online:
Published:
Contact:
Abstract:
Due to the operation simplicity of DPPH and ABTS+ radical scavenging arrays for evaluating antioxidant capacity and without a need for specific equipments, both assays have been widely chosen to evaluate antioxidant capacity. EC50 is generally used as a representative parameter for antioxidant capacity of antioxidants determined using DPPH· assay at 30 min of fixed reaction time or using ABTS+· assay at 6 min of fixed reaction time. The results of this investigation indicated that a big difference was made by the compositions and structures of antioxidants to reaction rates with DPPH·and ABTS+·free radicals. The reaction equilibrium time was also different so that wrong estimations might be achieved at the condition of fixed reaction time.
Key words: reaction time, DPPH radical scavenging array, ABTS+ radical scavenging array, antioxidant capacity evaluation
CLC Number:
TS202.3
LIN Lian-zhu,ZHAO Mou-ming*. Effect of Reaction Time on DPPH and ABTS+ Radical Scavenging Assays for Antioxidant Capacity Evaluation[J]. FOOD SCIENCE, 2010, 31(5): 63-67.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6300-201005015
https://www.spkx.net.cn/EN/Y2010/V31/I5/63