FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 194-196.doi: 10.7506/spkx1002-6300-201005043

Previous Articles     Next Articles

Optimization of Nutrient Factors in Medium for Lactobacillus fermentum Growth

YE Xue-fei，RUAN Hui，FENG Shi-kai，ZHU Cheng-liang，HE Guo-qing*   

  1. School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China
  • Received:2008-05-15 Revised:2009-11-27 Online:2010-03-01 Published:2010-12-29
  • Contact: HE Guo-qing E-mail:gqhe@zju.edu.cn

RichHTML

0

PDF (PC)

453

Abstract:

In this study, the optimal basal medium and the optimal carbon source, nitrogen source and growth factor in it were chosen for Lactobacilus fermentum growth, and the optimal addition amounts of the three nutrient factors were investigated using orthogonal array design. Results indicated that MRS medium was the most suitable for Lactobacilus fermentum growth, and its optimal formula consisted of 2.0% glucose, 0.5% beef extract, 0.5% casein peptone, 2% corn syrup, and other unchanged basic ingredients. The number of living Lactobacilus fermentum cells was over 1010 CFU/mL after 16 h to 18 h of cultivation in optimized MRS medium at 37 ℃, which exhibited a 10-fold increase compared with that in original MRS medium.

Key words: Lactobacilus fermentum La-Y1, nutrient factor, growth culture

CLC Number: