Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin

doi:10.7506/spkx1002-6300-201008015

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (8): 65-69.doi: 10.7506/spkx1002-6300-201008015

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Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin

HU Zhi-jun，HAO Li-jun，WANG Nan-xi，CHEN Jian-qiu*

Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China

Received:2009-05-21 Online:2010-04-15 Published:2010-12-29
Contact: CHEN Jian-qiu E-mail:cjqer@163.com

Abstract

Abstract:

To propose an optimal purification process of total flavonoids in 70% ethanol extract from citrus peel, the static and dynamic adsorption-desorption performance of D-101 macroporous adsorption resin towards total flavonoids from citrus peel was investigated. Major process parameters including pH of sample solution (ethanol extract of citrus peel), sample solution volume/D-101 resin weight ratio (mL/g), standing time and kind and amount (desorption solvent volume/ D-101 resin weight ratio, mL/g) of desorption solvent affecting either adsorption ratio or desorption ratio of total flavonoids were evaluated and their optimal values were determined to be pH 4.43, 15:1, 90 min, 95% ethanol and 25:1, respectively. The method proved to be simple and feasible for purification of crude total flavonoids extracted from citrus peel.

Key words: citrus peel, total flavonoids, D-101 macroporous adsorption resin, static adsorption, dynamic adsorption

CLC Number:

O623.54

HU Zhi-jun，HAO Li-jun，WANG Nan-xi，CHEN Jian-qiu*. Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin[J]. FOOD SCIENCE, 2010, 31(8): 65-69.

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Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin

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