Orthogonal Array Design for Extraction Optimization of Protein from Camellia oleifera Seed Meal

doi:10.7506/spkx1002-6300-201008022

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (8): 102-105.doi: 10.7506/spkx1002-6300-201008022

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Orthogonal Array Design for Extraction Optimization of Protein from Camellia oleifera Seed Meal

DING Dan-hua1，PENG Guang-hua2，XIA Hui1，WAN Hui3，HE Dong-ping3,*

1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China；
2. Tibeten Bureau of Quality and Technical Supervision, Lhasa 850000, China；
3. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China

Received:2009-05-31 Revised:2009-11-19 Online:2010-04-15 Published:2010-12-29
Contact: HE Dong-ping3 E-mail:hedp123456@163.com

Abstract

Abstract:

The basic chemical composition of Camellia oleifera seed meal and the isoelectric point of Camellia oleifera seed meal protein were determined. According to the determination results, a method based on alkaline extraction followed by acid precipitation was proposed for extraction of protein from Camellia oleifera seed meal. Single factor and orthogonal array design methods were employed to examine the effects of alkaline extraction parameters including material particle size, material/liquid ratio, pH and extraction time and temperature on protein yield. Results indicated that the extraction for 80 min at pH 10 and a material/liquid ratio of 1:25 gave a maximum protein yield of 57.8% from material powder with particle sizes below 80 mesh.

Key words: Camellia cleifera seed meal, protein, extraction

CLC Number:

TQ93

DING Dan-hua1，PENG Guang-hua2，XIA Hui1，WAN Hui3，HE Dong-ping3,*. Orthogonal Array Design for Extraction Optimization of Protein from Camellia oleifera Seed Meal[J]. FOOD SCIENCE, 2010, 31(8): 102-105.

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Orthogonal Array Design for Extraction Optimization of Protein from Camellia oleifera Seed Meal

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