Abstract:

A sensitive and rapid HPLC-MS/MS method was developed for the determination of 10 Beta-adrenergic agonists residues in pork. Samples were subjected to extraction with acidified acetontrile, followed by solid phase extraction clean-up before HPLC-MS/MS analysis. The developed standard curves of 10 Beta-adrenergic agonists all displayed good linearity over the concentration range of 0.5－100μg/kg. Mean recovery rates of 6 replicates for Beta-adrenergic agonist in a negative pork sample spiked at three levels were between 72% and 87%. The limit of detection of the method ranged from 0.1 to 1μg/kg. This method proved to be of simplicity, high sensitivity and good selectivity and provided satisfying determination results. Therefore, its application deserves to be popularized among various food testing laboratories.

Key words: Beta-adrenergic agonists, pork, HPLC-MS/MS