Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs

doi:10.7506/spkx1002-6630-200902046

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (2 ): 208-210.doi: 10.7506/spkx1002-6630-200902046

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Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs

GE Qing-lian1，GE Qing-feng2，LIU Di1，LU Xiao-juan1，ZHU Yun-fen1，LIU Xue-xian1，CHEN Kuan-wei1

(1. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China；2. College of Tourism Cuisine,
Yangzhou University, Yangzhou 225002, China)

Received:2008-01-28 Revised:2008-03-22 Online:2009-01-15 Published:2010-12-29
Contact: GE Qing-lian1 E-mail:zsj10800@sina.com

Abstract

Abstract:

A simple catalytic spectrophotometic with enzyme method for rapid determination of cholesterol in eggs was developed. Sample was prepared by direct saponification, and then detemined with spectrophotometry at 500 nm after enzyme catalyzed reaction. The recovery of this method is above 90% with RSD below 4% and the linear range is 20 to 600 mg/ml. It was proved that the method is precise and accurate, and so it is suitable for rapid determination of cholesterol in eggs.

Key words: catalytic spectrophotometry with enzyme, cholesterol, egg

CLC Number:

TS253.7

GE Qing-lian1，GE Qing-feng2，LIU Di1，LU Xiao-juan1，ZHU Yun-fen1，LIU Xue-xian1，CHEN Kuan-wei1. Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs[J]. FOOD SCIENCE, 2009, 30(2 ): 208-210.

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Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs

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