Abstract:

In this study, sweet potato meal was chosen as the principal constitute of culture medium to provide carbon source for the growth of yeast (Saccharomyces cerevisiaes). The mutation of original yeast was induced by UV radiation. After primary screening, secondary screening and domestication, a mutant of high alcohol-producing yeast S604 was obtained via 60-s UV radiation and 4-round screening. Then the culture medium composition of the mutant strain was optimized by orthogonal test. The fermntation experiment results showed that the alcohol yield by the mutant cultured in the optimized medium is higher than that by the original strain FCC2129.

Key words: yeast, sweet potato meal, mutation, alcohol, orthogonal test