Abstract:

The content of total flavonoids in leaves of Ilex latifolia Thunb. were determined using aluminum chloride method and aluminum nitrate-sodium nitrite method. The experimental results showed that the determination results of content of total flavonoids by the two methods are different from each other. The analysis on mechanism of color development of two methods indicated that the aluminum chloride method is suitable for the determination of total flavonoids in leaves of Ilex latifolia Thunb.. The relative standard deviation of precision is 2.2%. The spike recovery rates are from 90.7% to 109.5% and their average value is 97.8%. The extraction process of total flavonoids from leaves of Ilex latifolia Thunb. was optimized by means of orthogonal test design. The results showed that the extraction rate of total flavonoids is 0.68% under the optimal conditions of ratio of extractant to raw material 12:1, taking 60% ethanol as extractant, extraction time 2 h and extraction times 2.

Key words: leaves of Ilex latifolia Thunb., total flavonoids, determination method, extraction process