Abstract:

In this study, the volatile oil of Cuminum cyminum L. was extracted by steam distillation method and the cuminal content in the volatile oil was analyzed using GC-MS. The Shimazu Column DB-5 (30 m×0.32 mm, 0.25 μm) was used. The injection temperature was 220 ℃, and the initial temperature of 40 ℃ was increased to 220 ℃ at the rising rate of 8 ℃/min. The helium flow rate, split ratio and injection volume were 1.2 ml/min, 100:1 and 0.5 μl, respectively. The results showed that there is good linear relationship between integral value (Y) of peak area and cuminal content (X) in the range of 7.75 to 69.75 mg/ ml, and the linear equation is Y = 3.34 × 108X － 3.373 × 106 (r =0.9989). The RSDs in precision, repeatability and stability experiments are 1.40%, 2.3% and 1.2%. The average spike recovery is 96.5% with the RSD of 1.8% (n=5). The method is simple and accurate with good reproducibility, therefore it can be used for quality control of Cuminum cyminum L..

Key words: Cuminum cyminum L. , essential oil , GC-MS , cuminal