Limulus Amoebocyte Lysate (LAL) Assay Coupled with Gram Negative Bacteria (GNB) Count for Detecting γ-Ray-irradiated Chicken

doi:10.7506/spkx1002-6630-200906057

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 243-245.doi: 10.7506/spkx1002-6630-200906057

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Limulus Amoebocyte Lysate (LAL) Assay Coupled with Gram Negative Bacteria (GNB) Count for Detecting γ-Ray-irradiated Chicken

HU Qun，ZOU Chun-ying，GUO Li-ping

(Daxie Entry-Exit Inspection and Quarantine Bureau, Ningbo 315812, China)

Received:2008-05-12 Revised:2008-09-06 Online:2009-03-15 Published:2010-12-29
Contact: ZOU Chun-ying E-mail:hengbibg-hest@163.com

Abstract

Abstract:

Objective: To explore the availability of LAL/GNB method for screening irradiated chicken. Methods: Every 50 g of saled diced chicken was packaged into a sample and then every 12 chicken samples were irradiated byγ-ray of 0, 2 and 6 kGy, respectively. The viable GNB number in chicken sample was estimated by GNB count method, and the total number of GNB was estimated according to bacterial endotoxin concentration. The difference in the two numebers was used to judge if chicken sample had been irradiated or not. Results: In the 6 kGy-dose group, all 12 samples were detected to be irradiated. In the 4 kGydose group, 8 samples were detected to be irradiated, except 4 samples. In the control group, all 12 samples were detected to be not irradiated. Conclusion: The LAL/GNB method is available for detecting irradiated chicken.

Key words: LAL assay, Gram negative bacteria, γ-ray-irradiated chicken

CLC Number:

TS251.55

HU Qun，ZOU Chun-ying，GUO Li-ping. Limulus Amoebocyte Lysate (LAL) Assay Coupled with Gram Negative Bacteria (GNB) Count for Detecting γ-Ray-irradiated Chicken[J]. FOOD SCIENCE, 2009, 30(6): 243-245.

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Limulus Amoebocyte Lysate (LAL) Assay Coupled with Gram Negative Bacteria (GNB) Count for Detecting γ-Ray-irradiated Chicken

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