Abstract:

With gelatin, pectin, alginate sodium, calcium chloride and chitosan as wall materials, Bifidobacterium bifidum microcapsules were prepared by emulsification and double-layer embedment method. The determination results showed the diameter of microcapsule particles is 10 to 30  μm, the viable cell count is more than 109 CFU/g, and the embedding rate reaches 82.24%. After stimulated gastric solution or bile acid treatment, the viable cell count of Bifidobacterium bifidum in microcapsule is more than 108 CFU/g, which indicates its high acid tolerance. After the treatment of stimulated intestinal solution for 15 min, almost all microcapsules disintegrate and the release rate reaches 95.81%. The classic accelerating test proved that the Bifidobacterium bifidum microcapsules have good stability for storage. After one-year storage at room temperature, the viable cell count is still over 108 CFU/g.

Key words: Bifidobacterium bifidum, emulsification method, microcapsule, viable cell count